TRANCE/TNFSF11/RANK L Antibody (12A380) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27402
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
CyTOF-ready, Flow (Cell Surface), Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Cited:
Flow (Cell Surface), Flow Cytometry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 12A380
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A bacterially expressed fusion protein containing amino acid residues 1-317 of mouse TRANCE was used as immunogen.
Localization
Flow Cytometry (Surface): please see Yoneda et al. 2004 for details. Immunohistochemistry-Frozen: please see Yoneda et al. 2004 for details.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for TRANCE/TNFSF11/RANK L Antibody (12A380) - Azide and BSA Free
Flow Cytometry: TRANCE/TNFSF11/RANK L Antibody (12A380) - Azide and BSA Free [NBP2-27402]
Flow Cytometry: TRANCE/TNFSF11/RANK L Antibody (12A380) - Azide Free [NBP2-27402] - Analysis using the Alexa Fluor (R) 488 conjugate. Staining of RANKL in untreated mouse splenocytes (green) and 72 hour ConA-stimulated mouse splenocytes (red) using this antibody. 1 ug of RANKL antibody was used for this assay.Western Blot: TRANCE/TNFSF11/RANK L Antibody (12A380)Azide and BSA Free [NBP2-27402]
Western Blot: TRANCE/TNFSF11/RANK L Antibody (12A380) - Azide Free [NBP2-27402] - analysis of recombinant RANKL (partial) probed with RANKL antibody at 1 ug/ml.Immunohistochemistry: TRANCE/TNFSF11/RANK L Antibody (12A380) - Azide and BSA Free [NBP2-27402]
Immunohistochemistry: TRANCE/TNFSF11/RANK L Antibody (12A380) - Azide Free [NBP2-27402] - Analysis of FFPE human tonsil using TRANCE (12A380) antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining was observed. Staining was performed by Histowiz. Image using the standard format of this product.Applications for TRANCE/TNFSF11/RANK L Antibody (12A380) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1ul/1 million cells
Immunohistochemistry-Frozen
1:10-1:2000
Immunohistochemistry-Paraffin
reported in scientific literature (PMID 18705798)
Western Blot
1-5ug/ml
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TRANCE/TNFSF11/RANK L
Long Name
TNF-related Activation-induced Cytokine
Alternate Names
CD254, ODF, OPGL, RANK L, RANKL, TNFSF11
Entrez Gene IDs
21943 (Mouse)
Gene Symbol
TNFSF11
Additional TRANCE/TNFSF11/RANK L Products
Product Documents for TRANCE/TNFSF11/RANK L Antibody (12A380) - Azide and BSA Free
Product Specific Notices for TRANCE/TNFSF11/RANK L Antibody (12A380) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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