TRANCE/TNFSF11/RANK L Antibody (12A668) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-56512
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Rat, Guinea Pig
Applications
Validated:
Chromatin Immunoprecipitation (ChIP), ELISA, Flow (Cell Surface), Immunoassay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Chemotaxis, ELISA, Flow (Cell Surface), IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 12A668
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A bacterially expressed fusion protein containing amino acid residues 1-317 of mouse TRANCE/TNFSF11/RANK L was used as immunogen.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for TRANCE/TNFSF11/RANK L Antibody (12A668) - BSA Free
Immunohistochemistry: TRANCE/TNFSF11/RANK L Antibody (12A668) - BSA Free [NB100-56512]
Immunohistochemistry: TRANCE/TNFSF11/RANK L Antibody (12A668) [NB100-56512] - MQDL detects EMT biomarkers in clinical prostate cancer. EMT biomarkers (EpCAM, N-Cad, and RANKL)were detected in a clinical primary prostate cancer specimen (Gleason score 6; 3+3) with documented bone metastasis. M denotes cells that completed EMT and E/M indicates cells undergoing partial EMT. 400x. Image collected and cropped by CiteAb from the following publication (https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0028670) licensed under a CC-BY license.Western Blot: TRANCE/TNFSF11/RANK L Antibody (12A668)BSA Free [NB100-56512]
Western Blot: TRANCE/TNFSF11/RANK L Antibody (12A668) [NB100-56512] - Analysis using Azide Free version of NB100-56512. Human lymph node lysate (35ug per lane, RIPA buffer). Band detected at ~35kDa and ~28kDa. (Expected MW of 35.5kDa according to NP_003692.1 and of 27.7kDa according to NP_143026.1)Immunocytochemistry/ Immunofluorescence: TRANCE/TNFSF11/RANK L Antibody (12A668) - BSA Free [NB100-56512]
Immunocytochemistry/Immunofluorescence: TRANCE/TNFSF11/RANK L Antibody (12A668) [NB100-56512] - TRANCE was detected in immersion fixed mouse splenocytes using anti-human/mouse/rat mouse monoclonal antibody (Catalog # NB100-56512) for 1 hour at room temperature. Cells were stained using NL557 (red) fluorescent anti-mouse secondary antibodies (Catalog # NL007) and counterstained with DAPI (blue).Applications for TRANCE/TNFSF11/RANK L Antibody (12A668) - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
1:10-1:500
ELISA
reported in scientific literature (PMID 15935726)
Immunocytochemistry/ Immunofluorescence
20 ug/ml
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
5 ug/ml
Western Blot
0.5-2 ug/ml
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TRANCE/TNFSF11/RANK L
Long Name
TNF-related Activation-induced Cytokine
Alternate Names
CD254, ODF, OPGL, RANK L, RANKL, TNFSF11
Entrez Gene IDs
21943 (Mouse)
Gene Symbol
TNFSF11
UniProt
Additional TRANCE/TNFSF11/RANK L Products
Product Documents for TRANCE/TNFSF11/RANK L Antibody (12A668) - BSA Free
Product Specific Notices for TRANCE/TNFSF11/RANK L Antibody (12A668) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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