TRANCE/TNFSF11/RANK L Antibody (12A668) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56593

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 12A668

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all TRANCE/TNFSF11/RANK L Antibodies »

Product Specifications

Immunogen

A bacterially expressed fusion protein containing amino acid residues 1-317 of mouse TRANCE was used as immunogen (NP_003692).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TRANCE/TNFSF11/RANK L Antibody (12A668) - Azide and BSA Free

Western Blot: TRANCE/TNFSF11/RANK L Antibody (12A668)Azide and BSA Free [NB100-56593]

Western Blot: TRANCE/TNFSF11/RANK L Antibody (12A668)Azide and BSA Free [NB100-56593]

Western Blot: TRANCE/TNFSF11/RANK L Antibody (12A668) - Azide Free [NB100-56593] - Analysis of human lymph node lysate (35ug per lane, RIPA buffer) at 0.5ug/ml. Band detected at ~35kDa and ~28kDa. (Expected MW of 35.5kDa according to NP_003692.1 and of 27.7kDa according to NP_143026.1)
Immunocytochemistry/ Immunofluorescence: TRANCE/TNFSF11/RANK L Antibody (12A668) - Azide and BSA Free [NB100-56593]

Immunocytochemistry/ Immunofluorescence: TRANCE/TNFSF11/RANK L Antibody (12A668) - Azide and BSA Free [NB100-56593]

Immunocytochemistry/Immunofluorescence: TRANCE/TNFSF11/RANK L Antibody (12A668) - Azide Free [NB100-56593] - TRANCE was detected in immersion fixed mouse splenocytes using anti-human/mouse/rat mouse monoclonal antibody (Catalog # NB100-56512) for 1 hour at room temperature. Cells were stained using NL557 (red) fluorescent anti-mouse secondary antibodies (Catalog # NL007) and counterstained with DAPI (blue). Image using the standard format of this product.
Immunohistochemistry-Paraffin: TRANCE/TNFSF11/RANK L Antibody (12A668) - Azide and BSA Free [NB100-56593]

Immunohistochemistry-Paraffin: TRANCE/TNFSF11/RANK L Antibody (12A668) - Azide and BSA Free [NB100-56593]

Immunohistochemistry-Paraffin: TRANCE/TNFSF11/RANK L Antibody (12A668) - Azide Free [NB100-56593] - Formalin-fixed, paraffin-embedded human lymph node probed with Trance antibody at 5 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
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Applications for TRANCE/TNFSF11/RANK L Antibody (12A668) - Azide and BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation

1:10-1:500. Use reported in scientific literature (PMID 25859979)

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Immunohistochemistry-Paraffin

1 - 5 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Sterile - filtered PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRANCE/TNFSF11/RANK L

TRANCE/OPGL/RANKL/ODF is a recently identified member of tumor necrosis factor family. TRANCE (TNF-related activation-induced cytokine receptor) or RANKL {receptor activator of NF-kB (RANK) ligand} has been implicated in interactions between T cells and dendritic cells (1-2). RANK ligand (RANKL/TRANCE) binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival (2). TRANCE/RANKL is also important in T and B-cell maturation (3). As OPGL/ODF (Osteoprotegerin Ligand/Osteoclast differentiation factor), the same protein can both activate mature osteoclasts and mediate osteoclastogenesis (4). OPGL/TRANCE deficient mice show severe osteoporesis and complete absence of osteoclasts as a result of lack of osteogenesis (3).

Long Name

TNF-related Activation-induced Cytokine

Alternate Names

CD254, ODF, OPGL, RANK L, RANKL, TNFSF11

Entrez Gene IDs

8600 (Human); 21943 (Mouse)

Gene Symbol

TNFSF11

Additional TRANCE/TNFSF11/RANK L Products

Product Documents for TRANCE/TNFSF11/RANK L Antibody (12A668) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TRANCE/TNFSF11/RANK L Antibody (12A668) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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