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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI6H10

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Summary for Twist-2 Antibody (OTI6H10) - Azide and BSA Free

Immunogen

Full length human recombinant protein of human TWIST2 (NP_476527) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

17.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Twist-2 Antibody (OTI6H10) - Azide and BSA Free

Western Blot: Twist-2 Antibody (OTI6H10)Azide and BSA Free [NBP2-74724]

Western Blot: Twist-2 Antibody (OTI6H10)Azide and BSA Free [NBP2-74724]

Western Blot: Twist-2 Antibody (OTI6H10) - Azide and BSA Free [NBP2-74724] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TWIST2 .

Applications for Twist-2 Antibody (OTI6H10) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Twist-2

Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Twist. It is thought that during osteoblast development this protein may inhibit osteoblast maturation and maintain cells in a preosteoblast phenotype.

Long Name

Twist Related Protein 2 [Drosophila]

Alternate Names

bHLHa39, DERMO1, Twist2

Gene Symbol

TWIST2

Additional Twist-2 Products

Product Documents for Twist-2 Antibody (OTI6H10) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Twist-2 Antibody (OTI6H10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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