Ubiquitin Antibody (1B4-UB)

Novus Biologicals, part of Bio-Techne | Catalog # NB600-776

Novus Biologicals, part of Bio-Techne
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NB600-776
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Key Product Details

Species Reactivity

Bovine

Applications

ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1B4-UB

Concentration

1.0 mg/ml

View all Ubiquitin Antibodies »

Product Specifications

Immunogen

Ubiquitin from bovine erythrocytes conjugated to bovine gamma globulin

Reactivity Notes

Predicted cross-reactivities: Human

Localization

Cytoplasmic and Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Ubiquitin Antibody (1B4-UB)

Western Blot: Ubiquitin Antibody (1B4-UB)BSA Free [NB600-776]

Western Blot: Ubiquitin Antibody (1B4-UB)BSA Free [NB600-776]

Western Blot: Ubiquitin Antibody (1B4-UB) [NB600-776] - GST-ubiquitin detected with Mouse anti-Ubiquitin.
Immunohistochemistry-Paraffin: Ubiquitin Antibody (1B4-UB) - BSA Free [NB600-776]

Immunohistochemistry-Paraffin: Ubiquitin Antibody (1B4-UB) - BSA Free [NB600-776]

Immunohistochemistry-Paraffin: Ubiquitin Antibody (1B4-UB) [NB600-776] - Staining of human tonsil.
Immunohistochemistry-Paraffin: Ubiquitin Antibody (1B4-UB) - BSA Free [NB600-776]

Immunohistochemistry-Paraffin: Ubiquitin Antibody (1B4-UB) - BSA Free [NB600-776]

Immunohistochemistry-Paraffin: Ubiquitin Antibody (1B4-UB) [NB600-776] - Human hypophysis.

Applications for Ubiquitin Antibody (1B4-UB)

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:10-1:50

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:500-1:1000

Immunohistochemistry-Paraffin

1:500-1:1000

Western Blot

1:100-1:1000
Application Notes
For Flow Cytometry: Membrane permeabilisation is required for this application. For IHC: This product does not require protein digestion pre-treatment of paraffin sections

Reviewed Applications

Read 1 review rated 4 using NB600-776 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ubiquitin

Ubiquitin is a highly conserved protein of approximately 8.5kDa molecular weight, which has a normal role in the targeting of proteins for proteolytic degradation. To perform this function, the protein to be degraded is first covalently attached to the C-terminus of ubiquitin, and the ubiquitinated complex is then recognized by a complex of degradative enzymes. Interestingly, ubiquitin also becomes covalently bonded to many types of pathological inclusions, which appear to be resistant to normal degradation. Therefore, ubiquitin antibodies are very useful for studies of these inclusions. For example, the neurofibrillary tangles and paired helical filaments diagnostic of Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease are all heavily ubiquitinated and can be readily visualized with ubiquitin antibodies.

Alternate Names

UBB

Entrez Gene IDs

7314 (Human); 281370 (Bovine)

Gene Symbol

UBB

UniProt

P0CG53 (Bovine), P62990 (Bovine)

Additional Ubiquitin Products

Product Documents for Ubiquitin Antibody (1B4-UB)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Ubiquitin Antibody (1B4-UB)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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