Ubiquitin Antibody (P4G7-H11)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-19306

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Hamster, Rabbit

Applications

Flow Cytometry, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # P4G7-H11

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Ubiquitin Antibodies »

Product Summary for Ubiquitin Antibody (P4G7-H11)

Immunogen

Native bovine Ubiquitin

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

Ubiquitin (P4G7-H11)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Ubiquitin Antibody (P4G7-H11)

Western Blot: Ubiquitin Antibody (P4G7-H11) [NBP1-19306]

Western Blot: Ubiquitin Antibody (P4G7-H11) [NBP1-19306]

Western Blot: Ubiquitin Antibody (P4G7-H11) [NBP1-19306] - Western blot analysis of Ubiquitin: Lane 1: MW marker, Lane 2: Ubiquitin, Lane 3: HeLa, Lane 4: RK-13, Lane 5: 3T3, Lane 6: PC-12, Lane 7: Mouse Brain, Lane 8: Rat Brain, Lane 9: MDBK
Immunohistochemistry: Ubiquitin Antibody (P4G7-H11) [NBP1-19306]

Immunohistochemistry: Ubiquitin Antibody (P4G7-H11) [NBP1-19306]

Immunohistochemistry: Ubiquitin Antibody (P4G7-H11) [NBP1-19306] - Immunohistochemistry analysis of human brain, cortex tissue stained with Ubiquitin, mAb (P4G7-H11) at 5-10ug/ml.
Flow Cytometry: Ubiquitin Antibody (P4G7-H11) [NBP1-19306]

Flow Cytometry: Ubiquitin Antibody (P4G7-H11) [NBP1-19306]

Flow Cytometry: Ubiquitin Antibody (P4G7-H11) [NBP1-19306] - Flow cytometry analysis of human hepatoma QGY cells analyzed by flow cytometry using isotype control antibody (left) or Ubiquitin Monoclonal Antibody (P4G7-H11) (right) at 10ug/mL.

Applications for Ubiquitin Antibody (P4G7-H11)

Application
Recommended Usage

Flow Cytometry

10 ug/ml

Western Blot

1:100-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS containing 50% glycerol

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Ubiquitin

Ubiquitin is a highly conserved protein of about 8.5 kDa molecular weight, which has an ATP dependent role in the targeting of proteins for proteolytic degradation. To perform this function, the protein to be degraded is first covalently attached to the C terminus of ubiquitin, and the ubiquitinated complex is then recognized by a complex of degradative enzymes. Interestingly, ubiquitin also becomes covalently bonded to many types of pathological inclusions, which appear to be resistant to normal degradation. Therefore, ubiquitin antibodies are very useful for studies of these inclusions. For example, the neurofibrillary tangles and paired helical filaments diagnostic of Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease are all heavily ubiquitinated and can be readily visualized with ubiquitin antibodies.

Alternate Names

UBB

Entrez Gene IDs

22190 (Mouse); 50522 (Rat)

Gene Symbol

RPS27A

Additional Ubiquitin Products

Product Documents for Ubiquitin Antibody (P4G7-H11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Ubiquitin Antibody (P4G7-H11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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