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UNC5H2/UNC5B Antibody (1A9)

Novus Biologicals, part of Bio-Techne | Catalog # H00219699-M01

Novus Biologicals, part of Bio-Techne
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H00219699-M01

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Sandwich ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1A9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

UNC5B (NP_734465, 27 a.a. ~ 126 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GTDSGSEVLPDSFPSAPAEPLPYFLQEPQDAYIVKNKPVELRCRAFPATQIYFKCNGEWVSQNDHVTQEGLDEATGLRVREVQIEVSRQQVEELFGLEDY

Reactivity Notes

Human. Other species not tested.

Localization

Membrane; Single-pass type I membrane protein.

Specificity

UNC5B - unc-5 homolog B (C. elegans)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for UNC5H2/UNC5B Antibody (1A9)

Sandwich ELISA: UNC5H2/UNC5B Antibody (1A9) [H00219699-M01] - Detection limit for recombinant GST tagged UNC5B is approximately 1ng/ml as a capture antibody.

Applications for UNC5H2/UNC5B Antibody (1A9)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: UNC5H2/UNC5B

UNC5B belongs to a family of netrin 1 receptors thought to mediate the chemorepulsive effect of netrin 1 on specific axons. Unc5B is required for axon guidance. It mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand.

Long Name

Netrin Receptor UNC5B

Alternate Names

UNC5B

Entrez Gene IDs

219699 (Human)

Gene Symbol

UNC5B

OMIM

607870 (Human)

UniProt

Additional UNC5H2/UNC5B Products

Product Documents for UNC5H2/UNC5B Antibody (1A9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for UNC5H2/UNC5B Antibody (1A9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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