VAMP-8 Antibody (JF0963)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-66777

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-66777
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JF0963

Concentration

1 mg/ml

View all VAMP-8 Antibodies »

Product Summary for VAMP-8 Antibody (JF0963)

Immunogen

Synthetic peptide within Human VAMP-8 aa1-29 / 100. (SwissProt: Q9BV40 Human; SwissProt: O70404 Mouse; SwissProt: Q9WUF4 Rat)

Localization

Lysosome membrane, Early endosome membrane, Late endosome membrane, Cell membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VAMP-8 Antibody (JF0963)

Western Blot: VAMP-8 Antibody (JF0963) [NBP2-66777]

Western Blot: VAMP-8 Antibody (JF0963) [NBP2-66777]

Western Blot: VAMP-8 Antibody (JF0963) [NBP2-66777] - Western blot analysis of VAMP-8 on U93 cell lysates using anti-VAMP-8 antibody at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence: VAMP-8 Antibody (JF0963) [NBP2-66777]

Immunocytochemistry/ Immunofluorescence: VAMP-8 Antibody (JF0963) [NBP2-66777]

Immunocytochemistry/Immunofluorescence: VAMP-8 Antibody (JF0963) [NBP2-66777] - Staining VAMP8 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: VAMP-8 Antibody (JF0963) [NBP2-66777]

Immunohistochemistry-Paraffin: VAMP-8 Antibody (JF0963) [NBP2-66777]

Immunohistochemistry-Paraffin: VAMP-8 Antibody (JF0963) [NBP2-66777] - Analysis of paraffin-embedded mouse colon tissue using anti-VAMP8 antibody. Counter stained with hematoxylin.
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Applications for VAMP-8 Antibody (JF0963)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:1000-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VAMP-8

Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. It is associated with the perinuclear vesicular structures of the early endocytic compartment. It has been found that VAMP8 interacts specifically with the soluble NSF-attachment protein (alpha-SNAP), most likely through an VAMP8-containing SNARE complex.

Long Name

Vesicle-Associated Membrane Protein 8

Alternate Names

EDB, Endobrevin, VAMP8

Gene Symbol

VAMP8

Additional VAMP-8 Products

Product Documents for VAMP-8 Antibody (JF0963)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for VAMP-8 Antibody (JF0963)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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