Vinculin Antibody (VCL/2573)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-79807
![Immunohistochemistry-Paraffin: Vinculin Antibody (VCL/2573) [NBP2-79807]](https://resources.bio-techne.com/images/products/Vinculin-Antibody-VCL-2573-Immunohistochemistry-Paraffin-NBP2-79807-img0001.jpg "Immunohistochemistry-Paraffin: Vinculin Antibody (VCL/2573) [NBP2-79807]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # VCL/2573
Concentration
0.2 mg/ml
Product Summary for Vinculin Antibody (VCL/2573)
Immunogen
Recombinant fragment (around aa 174-322) of human Vinculin (VCL) protein (exact sequence is proprietary) (Uniprot: P18206)
Localization
Cytoplasmic
Marker
Marker of Age-related Macular Degeneration
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
117 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79941)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Vinculin Antibody (VCL/2573)
Immunohistochemistry-Paraffin: Vinculin Antibody (VCL/2573) [NBP2-79807]
Immunohistochemistry-Paraffin: Vinculin Antibody (VCL/2573) [NBP2-79807] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Vinculin Antibody (VCL/2573).![Protein Array: Vinculin Antibody (VCL/2573) [NBP2-79807]](https://resources.bio-techne.com/images/products/Vinculin-Antibody-VCL-2573-Protein-Array-NBP2-79807-img0002.jpg "Protein Array: Vinculin Antibody (VCL/2573) [NBP2-79807]")
Protein Array: Vinculin Antibody (VCL/2573) [NBP2-79807]
Protein Array: Vinculin Antibody (VCL/2573) [NBP2-79807] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Vinculin Antibody (VCL/2573) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5Applications for Vinculin Antibody (VCL/2573)
Application
Recommended Usage
Immunohistochemistry
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Vinculin
Additional Vinculin Products
Product Documents for Vinculin Antibody (VCL/2573)
Product Specific Notices for Vinculin Antibody (VCL/2573)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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