Vinculin Antibody (VCL/2573) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-79941
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # VCL/2573
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for Vinculin Antibody (VCL/2573) - Azide and BSA Free
Immunogen
Recombinant fragment (around aa 174-322) of human Vinculin (VCL) protein (exact sequence is proprietary) (Uniprot: P18206)
Localization
Cytoplasmic
Marker
Marker of Age-related Macular Degeneration
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
117 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-79807).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Vinculin Antibody (VCL/2573) - Azide and BSA Free
Immunohistochemistry-Paraffin: Vinculin Antibody (VCL/2573) - Azide and BSA Free [NBP2-79941]
Immunohistochemistry-Paraffin: Vinculin Antibody (VCL/2573) - Azide and BSA Free [NBP2-79941] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Vinculin Mouse Monoclonal Antibody (VCL/2573).Protein Array: Vinculin Antibody (VCL/2573)Azide and BSA Free [NBP2-79941]
Protein Array: Vinculin Antibody (VCL/2573) - Azide and BSA Free [NBP2-79941] - Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (mAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.Applications for Vinculin Antibody (VCL/2573) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
1 - 2 ug/mL
Immunohistochemistry-Paraffin
1 - 2 ug/mL
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Vinculin
Long Name
Metavinculin
Alternate Names
MVCL, VCL
Gene Symbol
VCL
Additional Vinculin Products
Product Documents for Vinculin Antibody (VCL/2573) - Azide and BSA Free
Product Specific Notices for Vinculin Antibody (VCL/2573) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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