Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 730823

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all VISTA/B7-H5/PD-1H Antibodies »

Product Specifications

Immunogen

Partial recombinant human VISTA/B7-H5 (amino acids 33-194) [UniProt Q9H7M9]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for VISTA/B7-H5/PD-1H Antibody (730823) - Azide and BSA Free

Flow Cytometry: VISTA/B7-H5/PD-1H Antibody (730823) - Azide and BSA Free [MAB71263]

Flow Cytometry: VISTA/B7-H5/PD-1H Antibody (730823) - Azide and BSA Free [MAB71263]

Flow Cytometry: VISTA/B7-H5/PD-1H Antibody (730823) [MAB71263] - A cell surface stain was performed on U-937 cells with VISTA/B7-H5/PD-1H (730823) antibody MAB71263PE (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.

Applications for VISTA/B7-H5/PD-1H Antibody (730823) - Azide and BSA Free

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is Cytof ready.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VISTA/B7-H5/PD-1H

Platelet Receptor Gi24, also known as Dies1 (Differentiation of ESC-1), VISTA (V-domain Ig suppressor of T cell activation), B7-H5, SISP1, and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. On ESCs, Gi24 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state. On tumor cells, Gi24 both promotes MT1-MMP expression and activity and serves as a substrate for MT1-MMP. This increases the potential for cell motility. Mature human Gi24 contains a 162 aa extracellular region with one V-type Ig-like domain and a 96 aa cytoplasmic domain. Human Gi24 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment. Over aa 33-194, human Gi24 shares 70% and 67% aa identity with mouse and rat Gi24, respectively.

Alternate Names

4632428N05Rik, B7-H5, C10orf54, Dies1, Gi24, PD-1H, PD1H, SISP1, VSIR

Gene Symbol

VSIR

Additional VISTA/B7-H5/PD-1H Products

Product Documents for VISTA/B7-H5/PD-1H Antibody (730823) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for VISTA/B7-H5/PD-1H Antibody (730823) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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