Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry

Label

PE (Excitation = 488 nm, Emission = 575 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 730823

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all VISTA/B7-H5/PD-1H Antibodies »

Product Specifications

Immunogen

Partial recombinant human VISTA/B7-H5 (amino acids 33-194) [UniProt Q9H7M9]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

>90% by SDS PAGE

Scientific Data Images for VISTA/B7-H5/PD-1H Antibody (730823) [PE]

Flow Cytometry: VISTA/B7-H5/PD-1H Antibody (730823) [PE] [MAB71263PE] - A cell surface stain was performed on U-937 cells with VISTA/B7-H5/PD-1H (730823) antibody MAB71263PE (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.

Applications for VISTA/B7-H5/PD-1H Antibody (730823) [PE]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: VISTA/B7-H5/PD-1H

VISTA, also known as B7-H5, PD-1H, platelet receptor Gi24, Dies1, and SISP1, is a transmembrane glycoprotein that promotes both MT1-MMP expression and the MT1-MMP mediated activation of MMP-2. VISTA supports the differentiation of embryonic stem cells (ESC) and enhances BMP-4 induced signaling in ESC but is also downregulated following BMP-4 exposure. It binds to BMP-4 directly and also associates with the type I BMP receptor Activin RIB/ALK-4. VISTA is expressed on the surface of naïve CD4+ T cells, regulatory T cells, and adipocytes. It is upregulated in vivo on activated monocytes and dendritic cells. VISTA inhibits CD4+ and CD8+ T cell proliferation and their production of IL-2 and IFN-gamma. Its expression on tumor cells attenuates the anti-tumor immune response and enables more rapid tumor progression. In contrast, VISTA limits disease progression in the autoimmune disease model EAE.

Alternate Names

4632428N05Rik, B7-H5, C10orf54, Dies1, Gi24, PD-1H, PD1H, SISP1, VSIR

Gene Symbol

VSIR

Additional VISTA/B7-H5/PD-1H Products

Product Documents for VISTA/B7-H5/PD-1H Antibody (730823) [PE]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for VISTA/B7-H5/PD-1H Antibody (730823) [PE]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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