WNK4 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB600-284

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Electron Microscopy, ICC/IF (Negative), Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Electron Microscopy, IF/IHC, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all WNK4 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to a region of the human WNK4 protein between residues 200-300. [UniProt# Q96J92]

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34448462).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for WNK4 Antibody - BSA Free

Western Blot: WNK4 AntibodyBSA Free [NB600-284]

Western Blot: WNK4 AntibodyBSA Free [NB600-284]

Western Blot: WNK4 Antibody [NB600-284] - Western Blot Image of anti-WNK4. Whole cell protein from Hek293 was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-WNK4 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence

Applications for WNK4 Antibody - BSA Free

Application
Recommended Usage

Electron Microscopy

reported in scientific literature (PMID 24434184)

Immunohistochemistry-Frozen

reported in scientific literature

Immunohistochemistry-Paraffin

reported in scientific literature (PMID 24434184)

Immunoprecipitation

reported in scientific literature (PMID 24434184)

Western Blot

2 ug/ml
Application Notes
In Western blot, a band is seen at ~135-155 kDa. It is not useful for Immunocytochemistry/Immunofluorescence.

Reviewed Applications

Read 1 review rated 4 using NB600-284 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: WNK4

WNK4 (protein kinase with no lysine 4) is a novel serine/threonine kinase which plays critical role in regulation of electrolyte homeostasis, signal transduction, survival and proliferation. WNK4 is highly expressed in kidney, colon and skin where it forms a part of tight junction complex and interacts with/activate several proteins including SCNN1A, SCNN1B, SCNN1D, SGK1, TRPV5, NEDD4L and TRPV6. It control SLC12A3 (thiazide-sensitive Na-Cl cotransporter) activity by phosphorylation process that prevents membrane trafficking of SLC12A3. WNK4 inhibits KCNJ1 (renal K+ channel) via a kinase-independent action leading to induction of protein clearance from the cell surface by clathrin-dependent endocytosis. WNK4 acts as a molecular switch that controls NaCl reabsorption and K+ secretion for maintaining integrated homeostasis. Autophosphorylation of Ser-335 is essential for WNK4's activity whereas phosphorylation of Ser-331 also increase its activity. Mutations of WNK4 can cause pseudohypoaldosteronism type alpha (PHA alpha), an autosomal dominant disease characterized by hyperkalemia, metabolic acidosis and hypertension.

Alternate Names

EC 2.7.11, EC 2.7.11.1, PHA2B, PRKWNK4protein kinase, lysine deficient 4, Protein kinase lysine-deficient 4, Protein kinase with no lysine 4, serine/threonine-protein kinase WNK4, WNK lysine deficient protein kinase 4

Gene Symbol

WNK4

Additional WNK4 Products

Product Documents for WNK4 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for WNK4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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