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WNK4 Antibody [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NB600-284CL1

Novus Biologicals, part of Bio-Techne
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NB600-284CL1

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Electron Microscopy, ICC/IF (Negative), Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

CoraFluor 1

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for WNK4 Antibody [CoraFluor™ 1]

Immunogen

A synthetic peptide made to a region of the human WNK4 protein between residues 200-300. [UniProt# Q96J92]

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34448462).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for WNK4 Antibody [CoraFluor™ 1]

Application
Recommended Usage

Electron Microscopy

Optimal dilutions of this antibody should be experimentally determined.

ICC/IF (Negative)

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: WNK4

WNK lysine deficient protein kinase 4 (WNK4) is a cytoplasmic serine-threonine kinase expressed in the distal nephron which likely plays an important role in hypertension. The role of WNK4 in kidney physiology is to act as a molecular switch between NaCl reabsorption (volume retention) and K+ secretion (waste release).

WNK4 mutations have been associated with an autosomal dominant disease called pseudohypoaldosteronism type II.

Alternate Names

EC 2.7.11, EC 2.7.11.1, PHA2B, PRKWNK4protein kinase, lysine deficient 4, Protein kinase lysine-deficient 4, Protein kinase with no lysine 4, serine/threonine-protein kinase WNK4, WNK lysine deficient protein kinase 4

Gene Symbol

WNK4

Additional WNK4 Products

Product Documents for WNK4 Antibody [CoraFluor™ 1]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for WNK4 Antibody [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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