WNK4 Antibody [DyLight 405]

Novus Biologicals, part of Bio-Techne | Catalog # NB600-284V

Submit a review

Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NB600-284AF350

Alexa Fluor 405

NB600-284AF405

Alexa Fluor 488

NB600-284AF488

Alexa Fluor 532

NB600-284AF532

Alexa Fluor 594

NB600-284AF594

Alexa Fluor 647

NB600-284AF647

Alexa Fluor 700

NB600-284AF700

Alexa Fluor 750

NB600-284AF750

Allophycocyanin

NB600-284APC

Biotin

NB600-284B

CoraFluor 1

NB600-284CL1

DyLight 350

NB600-284UV

DyLight 488

NB600-284G

DyLight 550

NB600-284R

DyLight 594

NB600-284DL594

DyLight 650

NB600-284C

DyLight 680

NB600-284FR

DyLight 755

NB600-284IR

FITC

NB600-284F

HRP

NB600-284H

Janelia Fluor 525

NB600-284JF525

Janelia Fluor 549

NB600-284JF549

Janelia Fluor 585

NB600-284JF585

Janelia Fluor 635

NB600-284JF635

Janelia Fluor 646

NB600-284JF646

Janelia Fluor 669

NB600-284JF669

mFluor Violet 450 SE

NB600-284MFV450

mFluor Violet 500 SE

NB600-284MFV500

mFluor Violet 610 SE

NB600-284MFV610

PE

NB600-284PE

PerCP

NB600-284PCP

Forumulation

Catalog #

BSA Free

NB600-284

Product Summary
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Electron Microscopy, ICC/IF (Negative), Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

DyLight 405 (Excitation = 400 nm, Emission = 420 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all WNK4 Antibodies »

Product Summary for WNK4 Antibody [DyLight 405]

Immunogen

A synthetic peptide made to a region of the human WNK4 protein between residues 200-300. [UniProt# Q96J92]

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34448462).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for WNK4 Antibody [DyLight 405]

Application

Recommended Usage

Electron Microscopy

Optimal dilutions of this antibody should be experimentally determined.

ICC/IF (Negative)

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: WNK4

WNK4 (protein kinase with no lysine 4) is a novel serine/threonine kinase which plays critical role in regulation of electrolyte homeostasis, signal transduction, survival and proliferation. WNK4 is highly expressed in kidney, colon and skin where it forms a part of tight junction complex and interacts with/activate several proteins including SCNN1A, SCNN1B, SCNN1D, SGK1, TRPV5, NEDD4L and TRPV6. It control SLC12A3 (thiazide-sensitive Na-Cl cotransporter) activity by phosphorylation process that prevents membrane trafficking of SLC12A3. WNK4 inhibits KCNJ1 (renal K+ channel) via a kinase-independent action leading to induction of protein clearance from the cell surface by clathrin-dependent endocytosis. WNK4 acts as a molecular switch that controls NaCl reabsorption and K+ secretion for maintaining integrated homeostasis. Autophosphorylation of Ser-335 is essential for WNK4's activity whereas phosphorylation of Ser-331 also increase its activity. Mutations of WNK4 can cause pseudohypoaldosteronism type alpha (PHA alpha), an autosomal dominant disease characterized by hyperkalemia, metabolic acidosis and hypertension.

Alternate Names

EC 2.7.11, EC 2.7.11.1, PHA2B, PRKWNK4protein kinase, lysine deficient 4, Protein kinase lysine-deficient 4, Protein kinase with no lysine 4, serine/threonine-protein kinase WNK4, WNK lysine deficient protein kinase 4

Gene Symbol

WNK4

Additional WNK4 Products

Product Documents for WNK4 Antibody [DyLight 405]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for WNK4 Antibody [DyLight 405]

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews