Wnt-3a Antibody (3A6) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-19050
![Western Blot: Wnt-3a Antibody (3A6)BSA Free [NBP1-19050]](https://resources.bio-techne.com/images/products/Wnt-3a-Antibody-3A6-Western-Blot-NBP1-19050-img0007.jpg "Western Blot: Wnt-3a Antibody (3A6)BSA Free [NBP1-19050]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 3A6
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Recombinant human Wnt-3a (19-352aa) purified from E. coli (NP_149122).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for Wnt-3a Antibody (3A6) - BSA Free
Western Blot: Wnt-3a Antibody (3A6)BSA Free [NBP1-19050]
Western Blot: Wnt-3a Antibody (3A6) [NBP1-19050] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with Wnt-3a antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1: 293T cell lysateLane 2: Wnt3a Transfected 293T cell lysate![Immunohistochemistry-Paraffin: Wnt-3a Antibody (3A6) - BSA Free [NBP1-19050]](https://resources.bio-techne.com/images/products/Wnt-3a-Antibody-3A6-Immunohistochemistry-Paraffin-NBP1-19050-img0004.jpg "Immunohistochemistry-Paraffin: Wnt-3a Antibody (3A6) - BSA Free [NBP1-19050]")
Immunohistochemistry-Paraffin: Wnt-3a Antibody (3A6) - BSA Free [NBP1-19050]
Immunohistochemistry-Paraffin: Wnt-3a Antibody (3A6) [NBP1-19050] - Human breast cancer tissue were incubated with anti-human Wn-t3a for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).![Western Blot: Wnt-3a Antibody (3A6)BSA Free [NBP1-19050]](https://resources.bio-techne.com/images/products/Wnt-3a-Antibody-3A6-Western-Blot-NBP1-19050-img0006.jpg "Western Blot: Wnt-3a Antibody (3A6)BSA Free [NBP1-19050]")
Western Blot: Wnt-3a Antibody (3A6)BSA Free [NBP1-19050]
Western Blot: Wnt-3a Antibody (3A6) [NBP1-19050] - The lysates of A549 (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Wnt3a (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Applications for Wnt-3a Antibody (3A6) - BSA Free
Application
Recommended Usage
ELISA
1:1000-1:2000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:50-1:100SDS-PAGE 1:100-1:2000
Western Blot
1:1000-1:2000
Application Notes
Use in ICC/IF reported in scientific literature (PMID 26708597)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 10% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Wnt-3a
Long Name
Wingless-type MMTV Integration Site Family, Member 3a
Alternate Names
Wnt3a
Entrez Gene IDs
89780 (Human)
Gene Symbol
WNT3A
UniProt
Additional Wnt-3a Products
Product Documents for Wnt-3a Antibody (3A6) - BSA Free
Product Specific Notices for Wnt-3a Antibody (3A6) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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