ZAP70 Antibody (ZAP70/4040R)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07223

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # ZAP70/4040R

Concentration

0.2 mg/ml

View all ZAP70 Antibodies »

Product Summary for ZAP70 Antibody (ZAP70/4040R)

Immunogen

Synthetic peptide corresponding to residues withinaa200-350 of ZAP70 was used as an immunogen. (Uniprot: P43403 )

Localization

Cell surface and cytoplasm

Marker

Chronic Lymphocytic Leukemia Marker

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 1 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08968)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for ZAP70 Antibody (ZAP70/4040R)

Immunohistochemistry-Paraffin: ZAP70 Antibody (ZAP70/4040R) [NBP3-07223]

Immunohistochemistry-Paraffin: ZAP70 Antibody (ZAP70/4040R) [NBP3-07223]

Immunohistochemistry-Paraffin: ZAP70 Antibody (ZAP70/4040R) [NBP3-07223] - Formalin-fixed, paraffin-embedded human tonsil stained with ZAP70 Recombinant Rabbit Monoclonal Antibody (ZAP70/4040R).
Immunohistochemistry-Paraffin: ZAP70 Antibody (ZAP70/4040R) [NBP3-07223]

Immunohistochemistry-Paraffin: ZAP70 Antibody (ZAP70/4040R) [NBP3-07223]

Immunohistochemistry-Paraffin: ZAP70 Antibody (ZAP70/4040R) [NBP3-07223] - Formalin-fixed, paraffin-embedded human tonsil stained with ZAP70 Recombinant Rabbit Monoclonal Antibody (ZAP70/4040R).

Applications for ZAP70 Antibody (ZAP70/4040R)

Application
Recommended Usage

ELISA

2-4 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

1 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: ZAP70

Zap-70, a Syk-family protein tyrosine kinase, plays a critical role in mediating T cell signal transduction in response to T cell receptor (TCR) activation (1). TCR-mediated activation of the Src-family kinases, Lck and Fyn, results in tyrosine phosphorylation of the TCR zeta and CD3 chains. These domains serve as targets for binding of ZAP-70 via its tandem SH2 domains. This binding correlates with activation of ZAP-70, a critical event in T cell activation (2). Following TCR engagement, ZAP-70 is phosphorylated on several tyrosine residues, presumably by two mechanisms: an autophosphorylation and a trans-phosphorylation by the Src-family tyrosine kinase, Lck. Phosphorylation of Tyr319 is required for full activation and increased positive downstream regulation by ZAP-70 (3).

Long Name

Zeta-chain [TCR] Associated Protein Kinase 70 kDa

Alternate Names

mrtle, mur, SRK, STD, TZK

Gene Symbol

ZAP70

UniProt

P43403

Additional ZAP70 Products

Product Documents for ZAP70 Antibody (ZAP70/4040R)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ZAP70 Antibody (ZAP70/4040R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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