ZAP70 Antibody (ZAP70/4040R) [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08968B

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Product Datasheet / COA / SDS

Recombinant Monoclonal Antibody

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Unconjugated

NBP3-07223

Alexa Fluor 350

NBP3-08968AF350

Alexa Fluor 405

NBP3-08968AF405

Alexa Fluor 488

NBP3-08968AF488

Alexa Fluor 532

NBP3-08968AF532

Alexa Fluor 594

NBP3-08968AF594

Alexa Fluor 647

NBP3-08968AF647

Alexa Fluor 700

NBP3-08968AF700

Alexa Fluor 750

NBP3-08968AF750

Allophycocyanin

NBP3-08968APC

CoraFluor 1

NBP3-08968CL1

DyLight 350

NBP3-08968UV

DyLight 405

NBP3-08968V

DyLight 488

NBP3-08968G

DyLight 550

NBP3-08968R

DyLight 594

NBP3-08968DL594

DyLight 650

NBP3-08968C

DyLight 680

NBP3-08968FR

DyLight 755

NBP3-08968IR

FITC

NBP3-08968F

HRP

NBP3-08968H

Janelia Fluor 525

NBP3-08968JF525

Janelia Fluor 549

NBP3-08968JF549

Janelia Fluor 646

NBP3-08968JF646

Janelia Fluor 669

NBP3-08968JF669

PE

NBP3-08968PE

PerCP

NBP3-08968PCP

Forumulation

Catalog #

Azide and BSA Free

NBP3-08968

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry-Paraffin

Label

Biotin

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # ZAP70/4040R

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ZAP70 Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to residues withinaa200-350 of ZAP70 was used as an immunogen. (Uniprot: P43403 )

Localization

Cell surface and cytoplasm

Marker

Chronic Lymphocytic Leukemia Marker

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Applications for ZAP70 Antibody (ZAP70/4040R) [Biotin]

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: ZAP70

Zap-70, a Syk-family protein tyrosine kinase, plays a critical role in mediating T cell signal transduction in response to T cell receptor (TCR) activation (1). TCR-mediated activation of the Src-family kinases, Lck and Fyn, results in tyrosine phosphorylation of the TCR zeta and CD3 chains. These domains serve as targets for binding of ZAP-70 via its tandem SH2 domains. This binding correlates with activation of ZAP-70, a critical event in T cell activation (2). Following TCR engagement, ZAP-70 is phosphorylated on several tyrosine residues, presumably by two mechanisms: an autophosphorylation and a trans-phosphorylation by the Src-family tyrosine kinase, Lck. Phosphorylation of Tyr319 is required for full activation and increased positive downstream regulation by ZAP-70 (3).

Long Name

Zeta-chain [TCR] Associated Protein Kinase 70 kDa

Alternate Names

mrtle, mur, SRK, STD, TZK

Gene Symbol

ZAP70

Additional ZAP70 Products

Product Documents for ZAP70 Antibody (ZAP70/4040R) [Biotin]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for ZAP70 Antibody (ZAP70/4040R) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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