Zebrafish Tie-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB928
Key Product Details
Species Reactivity
Validated:
Zebrafish
Cited:
Fish - Danio rerio (Zebrafish)
Applications
Validated:
Blockade of Receptor-ligand Interaction, Western Blot
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 176222
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant zebrafish Tie-2
Val22-His741
Accession # O73791
Val22-His741
Accession # O73791
Specificity
Detects zebrafish Tie-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with human or mouse Tie‑1 and Tie‑2 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Zebrafish Tie-2 Antibody
Angiopoietin-2 binding to Tie-2 blocked by Zebrafish Tie-2 Antibody.
In a functional ELISA, Recombinant Human Angiopoietin-2 (Catalog # 623-AN) binds to immobilized Recombinant Zebrafish Tie-2 Fc Chimera (Catalog # 928-T2) coated at 4 µg/mL (100 µL/well) in a dose-dependent manner (orange line). Binding is blocked (green line) by increasing concentrations of Mouse Anti-Zebrafish Tie-2 Monoclonal Antibody (Catalog # MAB928). At 0.5 µg/mL, this antibody will block >90% of the binding.Applications for Zebrafish Tie-2 Antibody
Application
Recommended Usage
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.03-0.1 µg/mL of this antibody will block 50% of the binding of 25 ng/mL of Recombinant Human Angiopoietin-2 (Catalog # 623-AN) to immobilized Recombinant Zebrafish Tie-2 Fc Chimera (Catalog # 928‑T2) coated at 4 µg/mL (100 µL/well). At 0.5 μg/mL, this antibody will block >90% of the binding.
Western Blot
1 µg/mL
Sample: Recombinant Zebrafish Tie-2 Fc Chimera (Catalog # 928-T2)
Sample: Recombinant Zebrafish Tie-2 Fc Chimera (Catalog # 928-T2)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Tie-2
Tie-1/Tie (tyrosine kinase with Ig and EGF homology domains 1) and Tie-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis.
Zebrafish Tie-2 cDNA encodes a 1116 amino acid (aa) residue precursor protein shares 38% sequence homology with human Tie-2 in the extracellular domain. Two ligands, angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2), which bind Tie-2 with high-affinity have been identified. Ang2 has been reported to act as an antagonist for Ang1. Mice engineered to overexpress Ang2 or to lack Ang1 or Tie-2 display similar angiogenesis defects.
References
- Partanen, J. and D.J. Dumont (1999) Curr. Top. Microbiol. Immunol. 237:159.
- Takakura, N. et al. (1998) Immunity 9:677.
- Procopio, W. et al. (1999) J. Biol. Chem. 274:30196.
Long Name
Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 2
Alternate Names
CD202b, TEK, Tie2
Entrez Gene IDs
Gene Symbol
TEK
UniProt
Additional Tie-2 Products
Product Documents for Zebrafish Tie-2 Antibody
Product Specific Notices for Zebrafish Tie-2 Antibody
For research use only
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