Proteome Profiler Human Pluripotent Stem Cell Array Kit

A membrane-based antibody array for the simultaneous detection of 15 established stem cell markers.

Why Use an Antibody Array to Detect Multiple Stem Cell Markers in Parallel?

Examining the expression profile of stem cells can be expensive, time-consuming, and can require specialized equipment. For example, microarrays are expensive and require specialized equipment. Similar expression analyses can be achieved by performing multiple immunoprecipitaions and Western blots, although these techniques are also time-consuming.

To address this need, R&D Systems offers the Proteome Profiler Human Pluripotent Stem Cell Antibody Array Kit. This rapid, sensitive, and economical multiplex assay allows users to determine if their cells express markers of pluripotency consistent with undifferentiated cells or if the cells express markers consistent with differentiation towards lineages such as the trophectoderm, ectoderm, mesoderm, and endoderm. No specialized equipment is needed as data are obtained by chemiluminescence.

The Proteome Profiler Human Pluripotent Stem Cell Antibody Array Kit:

• Is a sensitive multiplex tool to verify stem cell identity using 15 stem cell markers.
• Has a protocol that can be completed with just 5.5 hours of hands-on time.
• Is more efficient than other techniques used to establish pluripotency.
• Does not require specialized equipment.

Each kit contains eight nitrocellulose membranes spotted with 15 different antibodies printed in duplicate to detect stem cell markers. Buffers, biotinylated detection antibodies, streptavidin-HRP, and chemiluminescent reagents are included to enable the detection of stem cell markers from cell lysates.

• 8-well Rectangular Multi-dish
• Human Pluripotent Stem Cell Array - 8 nitrocellulose membranes each spotted with 15 different antibodies to stem cell markers printed in duplicate
• Array Buffer 1
• Array Buffer 2 Concentrate (5X)
• Array Buffer 3
• Chemi Reagent 1
• Chemi Reagent 2
• Detection Antibody Cocktail, Human Pluripotent Stem Cell Array
• Lysis Buffer 16
• Streptavidin-HRP
• Transparency Overlay Template
• Wash Buffer Concentrate (25X)

Analytes Detected by this kit:

• Oct-3/4
• Nanog
• SOX2
• E-Cadherin
• Alpha-Fetoprotein (AFP)
• GATA-4
• HNF-3 beta/FoxA2
• PDX-1/IPF1
• SOX17
• Otx2
• TP63/TP73L
• Goosecoid (Gsc)
• Snail
• VEGF R2/KDR/Flk-1
• HCG

Stability and Storage

Store the unopened kit at 2 ^°C to 8 ^°C. Do not use past kit expiration date.

Embryonic stem (ES) cells are pluripotent stem cells derived from the inner cell mass of pre-implantation embryos. Induced pluripotent stem (iPS) cells can be generated by somatic cell reprogramming following the exogenous expression of specific transcription factors (Oct-3/4, KLF4, SOX2, and c-Myc). These cell types are capable of unlimited, undifferentiated proliferation in vitro and still maintain the capacity to differentiate into a wide variety of somatic cells. In this capacity, pluripotent stem cells have widespread clinical potential for the treatments of heart disease, diabetes, spinal cord injury, and a variety of neurodegenerative disorders.

R&D Systems offers a wide range of products to support pluripotent stem cell culture and differentiation. Mouse embryonic fibroblasts may be used to maintain and expand pluripotent stem cells in an undifferentiated state. We also offer defined culture media, which are specifically optimized for use with human or rodent pluripotent stem cells. In addition, R&D Systems offers a variety of products to assess differentiation status and identify specific stem cell types of interest, including panels of marker antibodies, primer pairs, multi-color flow cytometry kits, and specialized verification kits.