CellXVivo Mouse Th2 Cell Differentiation Kit

For the differentiation of Th2 cells from a preparation of CD4+ T cells isolated from mouse splenocytes.

Why Expand and Differentiate Th2 Cells Ex Vivo?

T helper type 2 (Th2) cells are a lineage of CD4⁺ effector T cells that provide host protection against intestinal parasites and extracellular bacteria. In addition, they provide support for B cell-dependent humoral responses. Pathological Th2 cell activity is a hallmark of allergic inflammation and asthma. Differentiation of CD4⁺ effector cells into the Th2 lineage is promoted by cytokines such as IL-4 in combination with either IL-2, IL-7, or TSLP. Th2 cells secrete IL-4, IL-5, IL-9, IL-13, and IL-17E/IL-25. In vitro differentiation of mouse Th2 cells from the larger naïve CD4⁺ T cell population provides increased numbers of Th2 cells to facilitate downstream research. The CellXVivo^™ Mouse Th2 Cell Differentiation Kit contains all necessary components to differentiate mouse naïve CD4⁺ T cells into Th2 polarized cells.

This kit contains the following reagents for the ex vivo differentiation of mouse Th2 cells.

• Hamster Anti-Mouse CD3, Th2
• Rat Anti-Mouse CD28, Th2
• Mouse Th2 Reagent 1
• Mouse Th2 Reagent 2
• Mouse Th2 Reagent 3
• Reconstitution Buffer 1
• Reconstitution Buffer 2
• 20X Wash Buffer

The quantity of the components in the kit is sufficient to differentiate approximately 4 x 10⁶ naïve CD4⁺ T cells, and generate 80 x 10⁶ T cells, of which >40% are IL-4⁺ Th2 polarized cells.

Note: Results may vary due to strain, age, and/or the health of the mice used for isolation.

Stability and Storage

Store the unopened kit at ≤-20 °C. Do not use past the kit expiration date.

Limitations

• FOR LABORATORY RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
• The safety and efficacy of this product in diagnostic or other clinical uses has not been established.
• This reagent should not be used beyond the expiration date indicated on the label.
• Results may vary due to variations among cells derived from different donors.