ExCellerate B Cell Media, Xeno-Free Best Seller
R&D Systems, part of Bio-Techne | Catalog # CCM031
Key Product Details
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Improved B Cell Expansion using ExCellerate B Cell Media. Human B cells were isolated from peripheral blood mononuclear cells (PBMCs) and cultured for 10 days in either ExCellerate B Cell Media or RPMI + 10% FBS. Both media conditions were supplemented with reagents included in the CellXVivo Human B Cell Expansion Kit (Catalog # CDK005). A) Fold expansion of B cells at 5 and 10 days in culture (n=3). B) Light microscopy of B cells at 20X magnification after 5 days of culture. |
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Detection of CD19 but not CD3 in Human B Cells. Human B Cells were cultured for 5 days in ExCellerate B Cell Media, supplemented with reagents included in the CellXVivo™ Human B Cell Expansion Kit (Catalog # CDK015). The cells were labeled with a Mouse Anti-Human CD19 PE-conjugated Antibody (Catalog # FAB4867P) and with a Mouse Anti-Human CD3 epsilon APC-conjugated (Catalog # FAB100A). CD19+CD3- B cells were observed (>97%) following this culture protocol. |
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Improved Positive Clone Isolation of B cells using ExCellerate B Cell Media. Rabbit B cells were isolated from PBMCs and plated into 384-well plates (1 cell/well) using either ExCellerate B Cell Media or RPMI + 10% FBS. Clonal growth was monitored and screening was performed to identify antigen-specific B cell clones using an ELISA-based protocol and with PCR-based monoclonal cloning of heavy and light chains. Positive identification of clones producing the correct antibody was >2.5 fold higher when cultured using ExCellerate B Cell Media compared to RPMI + 10% FBS (n=8). |
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Expansion of Mouse B Cells using ExCellerate B Cell Media. Mouse B cells were isolated from splenocytes using the MagCellect Mouse B Cell Isolation Kit (Catalog # MAGM204) and expanded using ExCellerate B Cell Media with or without Recombinant Mouse IL-4 and CD40 Ab. A) Expansion of mouse B cells was monitored using Rezasurin. Data show that Excellerate B Cell Media supplemented with IL-4 (Catalog # 404-ML) and CD40 Ab (Catalog # MAB440) results in robust mouse B cell expansion. B) Representative brightfield images of mouse B cell colonies. C) Expanded mouse B cells are B220+CD19+ (> 98%) and negative for both CD3 and CD4. |