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StemXVivo Rat Chondrogenic Supplement, 0.5 mL

R&D Systems, part of Bio-Techne | Catalog # CCM020

R&D Systems, part of Bio-Techne

Key Product Details

Detection of Aggrecan in a Rat MSC-differentiated Chondrogenic Pellet Section. 
(2)

Assay Procedure

Refer to the product datasheet for complete product details.

Briefly, rat MSCs are differentiated into chondrocytes using the following in vitro differentiation procedure:

  • Culture multipotent cells of interest
  • Induce chondrogenic differentiation using a media supplement
  • Evaluate differentiation using a mature phenotype marker antibody and fluorescent ICC

For use with Human/Mouse/Rat StemXVivo® Chondrogenic Base Media (Catalog # CCM005).

 

 

Reagents Provided

Reagents supplied in the Rat StemXVivo® Chondrogenic Supplement (Catalog # CCM020):

  • 0.5 mL of StemXVivo® Chondrogenic Supplement

 

Other Supplies Required

Reagents

  • Human/Mouse/Rat StemXVivo® Chondrogenic Base Media (Catalog # CCM005)
  • Penicillin-Streptomycin-Glutamate (100X)

Materials

  • MSCs
  • 15 mL centrifuge tubes
  • Pipettes and pipette tips
  • Serological pipettes

Equipment

  • 37 °C and 5% CO2 incubator
  • Centrifuge
  • Hemocytometer
  • Inverted microscope
  • 2 °C to 8 °C refrigerator
  • 37 °C water bath

 

Procedure Overview

This protocol has been tested using bone marrow- and/or adipose tissue-derived MSCs. If using a different tissue source or cell line, the protocol below may need to be optimized.

Osteogenic Differentiation

Transfer 2.5 x 105 MSCs to a 15 mL conical tube.

Centrifuge and resuspend the cells in Chondrogenic Differentiation Medium.

Centrifuge the cells but do not remove the medium.

Every 2-3 days, replace with fresh Chondrogenic Differentiation Medium.

After 14-21 days, the chondrogenic pellet can be harvested and analyzed.

Protocol for CCM020
 
Protocol for CCM020
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Product Documents for StemXVivo Rat Chondrogenic Supplement, 0.5 mL

Certificate of Analysis

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