StemXVivo Serum-Free Human T Cell Base Media

Pre-optimized for ex vivo culture of human T lymphocytes.

Why use pre-optimized media for ex vivo culture of human T lymphocytes?

Isolation of human T-lymphocytes from peripheral blood is a time consuming process that can yield cells of varying purity and quality.

Once in culture, the maintenance and expansion of T lymphocytes is highly dependent on the media, growth factors, and cytokines. R&D Systems offers Human StemXVivo^® Serum-Free T Cell Base Media, a defined media that outperforms RPMI containing Fetal Bovine Serum in its ability to support T cell expansion.

Human StemXVivo^® Serum-Free T Cell Base Media:

• Supports T lymphocyte expansion better than RPMI containing 10% FBS.
• Decreases experimental variability through the use of defined media components.
• Can be used for multiple applications including mixed lymphocyte reactions (MLR), expansion of antigen-specific or non-specific T cells, and proliferation of peripheral blood lymphocytes in the presence of pharmacological agents such as PMA or ConA

• 250 mL StemXVivo^® Serum-Free T Cell Base Media.

The quantity of each component in this kit is sufficient to differentiate two 24-well plates, or an equivalent surface area, of pluripotent stem cells into hepatocyte-like cells

Stability and Storage

Upon receipt, the Human StemXVivo^® Serum-Free T Cell Base Media should be stored at =-20° C in a manual defrost freezer. The media can be thawed at 2 °C to 8 °C or at room temperature. Thawed media can be aliquoted and stored at =-20 °C in a manual defrost freezer until the expiration date on the vial label or used within a month when stored in the dark at 2 °C to 8 °C. Avoid repeated freeze-thaw cycles.

Precautions

The human origin-derived components used in this product have been derived from human plasma, which has been tested and found negative for antibodies to HIV-1/2, hepatitis B surface antigen (HBsAg), and hepatitis C virus (HCV). However, the medium should be handled as if potentially infectious. Safe laboratory procedures should be followed and protective clothing should be worn when handling this media. The acute and chronic effects of over-exposure to this media are unknown.

Limitations

• FOR LABORATORY RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
• The safety and efficacy of this product in diagnostic or other clinical uses has not been established.
• This reagent should not be used beyond the expiration date indicated on the label.
• Results may vary due to variations among primary T lymphocyte populations derived from different donors.

The definitive hematopoietic system is made up of all adult blood cell types including megakaryocytes, erythrocytes, and cells of the myeloid and lymphoid lineages. All of these cells are derived from multipotent hematopoietic stem cells (HSCs) through a succession of precursors with progressively limited potential. Hematopoietic stem cells are tissue-specific stem cells that exhibit remarkable self-renewal capacity and are responsible for the life-long mainte­nance of the hematopoietic system. HSCs are rare cells that reside in adult bone marrow where hematopoiesis is continu­ously taking place. They can also be found in cord blood, fetal liver, adult spleen, and peripheral blood. R&D Systems offers several products for studying hematopoietic lineage cells including serum-free media, lineage deple­tion antibodies and kits, and reagents for performing colony forming cell (CFC) assays.