Rat Agrin ELISA Kit (Colorimetric)

Name: Rat Agrin ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP2-66663

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-66663

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

18.75 pg/mL

Assay Range

31.25 - 2000 pg/mL

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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat AGRN in samples. No significant cross-reactivity or interference between Rat AGRN and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.99%

Inter-Assay Precision (Precision between assays) CV% < 5.26%

Recovery for Rat Agrin ELISA Kit (Colorimetric)

Recovery

87-107%

Linearity

Scientific Data Images for Rat Agrin ELISA Kit (Colorimetric)

ELISA: Rat Agrin ELISA Kit (Colorimetric) [NBP2-66663] -

ELISA: Rat Agrin ELISA Kit (Colorimetric) [NBP2-66663] - Standard Curve Reference

Kit Contents for Rat Agrin ELISA Kit (Colorimetric)

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro ELISA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Stop Solution
Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Agrin

Agrin is an essential extracellular matrix component which promotes clustering of nicotinic acetylcholine receptors (nAChRs) and other proteins during development at the neuromuscular junction. Agrin, MuSK and Rapsyn are all essential components for AChR aggregation, through an unknown mechanism. The C-terminal region of agrin is released into the medium, interacts with receptors on the muscle surface and induces AChR aggregation. The central region contains two O-linked glycosylation sites and a domain homologous to domain III of laminin. The N-terminal region anchors agrin to the extracellular matrix via other basal membrane components. This region also contains a protease inhibitor domain and glycosaminoglycan attachment sites; increasing the predicted MW from 200kDa to ~600kDa.

Alternate Names

AGRN

Gene Symbol