Human AIF-1/Iba1 ELISA Kit (Chemiluminescence)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-66674
Key Product Details
Sample Type & Volume Required Per Well
Serum, plasma and other biological fluids (100 uL)
Sensitivity
7.50 pg/mL
Assay Range
12.50 - 800 pg/mL
Product Specifications
Assay Type
Sandwich-CLIA
Kit Type
ELISA Kit (Chemiluminescence)
Reactivity
Human
Specificity
No significant cross-reactivity or interference between Human AIF1 and analogues was observed for this Human AIF-1/Iba1 ELISA Kit (Chemiluminescence).
Precision
Intra-Assay Precision (Precision within an assay) %CV < 9.96
Inter-Assay Precision (Precision between assays) %CV < 9.6
Recovery for Human AIF-1/Iba1 ELISA Kit (Chemiluminescence)
Recovery
85 - 115%
Linearity
Scientific Data Images for Human AIF-1/Iba1 ELISA Kit (Chemiluminescence)
ELISA: Human AIF-1/Iba1 ELISA Kit (Chemiluminescence) [NBP2-66674] -
ELISA: Human AIF-1/Iba1 ELISA Kit (Chemiluminescence) [NBP2-66674] - Standard Curve ReferenceKit Contents for Human AIF-1/Iba1 ELISA Kit (Chemiluminescence)
- Biotinylated Detection Antibody (100X)
- Biotinylated Detection Antibody Diluent
- HRP Conjugate (100X)
- HRP Conjugate Diluent
- Microplate
- Plate Sealer
- Reference Standard
- Reference Standard & Sample Diluent
- Substrate Reagent A
- Substrate Reagent B
- Wash Buffer (25X)
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: AIF-1/Iba1
Several cellular functions have been associated with AIF-1/Iba1 expression including cell growth, cell migration, actin bundling, membrane ruffling, and phagocytic activity (2, 4). Iba1 induces Rac signaling through a PLC-gamma dependent pathway (1). Rac, a member of the Rho family of small GTPases, localizes with Iba1 and F-actin in membrane ruffles and phagocytic cups and plays a role in microglia activation. AIF-1/Iba1 induction in macrophages and microglia occur in association with immunological inflammatory processes in various disease states including endometriosis, cerebral infarction and rheumatoid arthritis (5). Immunodetection of Iba1 through flow cytometry, immunohistochemical or immunocytochemical applications is commonly used for identification and analysis of microglia.
References
1. Imai, Y., & Kohsaka, S. (2002). Intracellular signaling in M-CSF-induced microglia activation: Role of Iba1. GLIA. https://doi.org/10.1002/glia.10149
2. Deininger, M. H., Meyermann, R., & Schluesener, H. J. (2002). The allograft inflammatory factor-1 family of proteins. FEBS Letters. https://doi.org/10.1016/S0014-5793(02)02430-4
3. Utans, U., Quist, W. C., Mcmanus, B. M., Wilson, J. E., Arceci, R. J., Wallace, A. F., & Russell, M. E. (1996). Allograft inflammatory factor-1: A cytokine-responsive macrophage molecule expressed in transplanted human hearts. Transplantation. https://doi.org/10.1097/00007890-199605150-00018
4. Franco-Bocanegra, McAuley, Nicoll, & Boche. (2019). Molecular Mechanisms of Microglial Motility: Changes in Ageing and Alzheimer's Disease. Cells. https://doi.org/10.3390/cells8060639
5. Kimura, M., Kawahito, Y., Obayashi, H., Ohta, M., Hara, H., Adachi, T., ... Yoshikawa, T. (2007). A Critical Role for Allograft Inflammatory Factor-1 in the Pathogenesis of Rheumatoid Arthritis. The Journal of Immunology. https://doi.org/10.4049/jimmunol.178.5.3316
Long Name
Allograft Inflammatory Factor 1
Alternate Names
AIF1, IBA1, IRT1
Gene Symbol
AIF1
Additional AIF-1/Iba1 Products
Product Documents for Human AIF-1/Iba1 ELISA Kit (Chemiluminescence)
Product Specific Notices for Human AIF-1/Iba1 ELISA Kit (Chemiluminescence)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
Loading...
Loading...
Loading...
Loading...