HMGB1/HMG-1 Antibody Pair

Novus Biologicals, part of Bio-Techne | Catalog # H00003146-AP44

Novus Biologicals, part of Bio-Techne
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Key Product Details

Assay Type

Sandwich ELISA

Assay Range

3 ng/ml to 100 ng/ml

Reactivity

Human

View all HMGB1/HMG-1 ELISA Kits »

Product Specifications

Description

Quality control test: Standard curve using recombinant protein (H00003146-P01) as an analyte. This antibody pair set comes with matched antibody pair to detect and quantify protein level of human HMGB1.

Conjugate

Unconjugated

Application Notes

Reagents are sufficient for at least 3-5 x 96 well plates using recommended protocols.

Scientific Data Images for HMGB1/HMG-1 Antibody Pair

Sandwich ELISA: HMGB1 Antibody Pair [H00003146-AP44] - [H00003146-P01] - Standard curve using recombinant protein as an analyte.
Sandwich ELISA: HMGB1 Antibody Pair [H00003146-AP44] - Detection sensitivity ranging from 3 ng/ml to 100 ng/ml.

Kit Contents for HMGB1/HMG-1 Antibody Pair

  • Capture antibody: mouse monoclonal anti-HMGB1 IgG2a Kappa (100 ug)
  • Detection antibody: biotinylated mouse monoclonal anti-HMGB1 IgG1 Kappa (50 ug)

Preparation and Storage

Formulation

1x PBS, pH 7.4

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HMGB1/HMG-1

HMGB1 and HMGB2 are part of the chromatin non-histone high mobility group proteins 1 and 2. HMGB1 and HMGB2 (containing multiple HMG-boxes) are conserved domains of 80 amino acids which mediate the DNA binding of many proteins. HMG box domains recognize DNA structure. Both HMGB1 and HMGB2 contain a N-terminal HMG box, a central HMG box, and an acidic carboxy terminus. The acidic tails of HMGB1 and HMGB2 contain multiple serine residues which match the phosphorylation consensus sites of casein kinase II, and phosphorylation of this domain appears to be important for proper functioning of these proteins. HMGB1 and HMGB2 have been shown to facilitate the binding of various sequence-specific transcription factors to their respective DNA binding sites. HMGB1 and HMGB2 may also serve as architectural factors that recognize and mediate DNA structural changes that accompany various events such as DNA repair, transcription, and replication.

Long Name

High Mobility Group Protein 1

Alternate Names

HMG-1, HMG1

Gene Symbol

HMGB1

Additional HMGB1/HMG-1 Products

Product Documents for HMGB1/HMG-1 Antibody Pair

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HMGB1/HMG-1 Antibody Pair

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

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