Human ACE-2 DuoSet ELISA
R&D Systems, part of Bio-Techne | Catalog # DY933-05
Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
0.313-20 ng/mL
Sample Type
Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Human
Human ACE-2 DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Product Summary for Human ACE-2 DuoSet ELISA
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Angiotensin Converting Enzyme 2 (ACE-2). The suggested diluent is suitable for the analysis of most cell culture supernate, cell lysate, serum, plasma, and urine samples. Diluents for complex matrices, such astissue lysates, serum, plasma, and urine, should be evaluated prior to use in this DuoSet.
Product Specifications
Assay Format
96-well strip plate (sold separately)
Sample Volume Required
100 µL
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Scientific Data Images for Human ACE-2 DuoSet ELISA
Human ACE-2 Standard Curve
Kit Contents for Human ACE-2 DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
The components listed above may be purchased separately:
PBS:(Catalog #DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer:(Catalog #WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution:1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog #DY999)
Stop Solution:2 N H2SO4(Catalog #DY994)
Microplates: R&D Systems (Catalog #DY990), or equivalent
Plate Sealers:ELISA Plate Sealers (Catalog #DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product.
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: ACE-2
Angiotensin converting enzyme 2 (ACE-2), also known as ACEH (ACE homolog), is an integral membrane protein and a zinc metalloprotease of the ACE family. The predicted human ACE-2 protein sequence consists of 805 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 cleaves angiotensins I and II as a carboxypeptidase. ACE-2 has been shown to be a functional receptor of the human coronaviruses SARS-CoV and SARS-CoV-2 . Amongst ongoing research into the cellular entry mechanism of coronavirus via ACE-2, an anti-human ACE-2 antibody (R&D Systems Catalog # AF933) has blocked this interaction.
Long Name
Angiotensin I Converting Enzyme 2
Alternate Names
ACE2, ACEH
Entrez Gene IDs
Gene Symbol
ACE2
Additional ACE-2 Products
Product Documents for Human ACE-2 DuoSet ELISA
Product Specific Notices for Human ACE-2 DuoSet ELISA
For research use only
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