Human C-Reactive Protein/CRP Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DCRP00
Discontinued Product
DCRP00 has been discontinued.
An alternative/replacement product is available:
DCRP00B. View all C-Reactive Protein/CRP products.
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
Sensitivity
0.022 ng/mL
Assay Range
0.78-50 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human C-Reactive Protein/CRP Quantikine ELISA Kit
The Quantikine Human CRP Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human CRP in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human CRP and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human CRP showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human CRP.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human CRP
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Serum/Plasma - Samples from apparently healthy volunteers were evaluated for the presence
of human CRP in this assay. No medical histories were available for the donors used in this
study.
Note: Four additional serum, EDTA plasma, and heparin plasma patient sample sets were tested
and resulted in substantially higher values. The values for each donor averaged 7364 ng/mL,
12,363 ng/mL, 16,064 ng/mL, and 43,036 ng/mL.
| Sample Type | Mean (ng/mL) | Range (ng/mL) | Standard Deviation (ng/mL) |
| Serum (n=35) | 1769 | 109 | 1214 |
| EDTA plasma (n=35) | 1547 | 104-4185 | 1082 |
| Heparin plasma (n=35) | 1624 | 108-4523 | 1138 |
Cell Culture Supernates - Human peripheral blood cells (1 x 106
cells/mL) were cultured in
DMEM supplemented with 5% fetal bovine serum, 50 μM beta-mercaptoethanol,
2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were
cultured unstimulated or stimulated with 10 μg/mL PHA. Aliquots of the cell culture supernate
were removed and assayed for levels of human CRP. No detectable levels were observed.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 4.79 | 8.66 | 18.9 | 4.84 | 8.66 | 17.5 |
| Standard Deviation | 0.21 | 0.33 | 1.57 | 0.29 | 0.61 | 1.15 |
| CV% | 4.4 | 3.8 | 8.3 | 6.0 | 7.0 | 6.6 |
Recovery for Human C-Reactive Protein/CRP Quantikine ELISA Kit
The recovery of CRP spiked to levels throughout the range of the assay in cell culture media was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 100 | 92-110 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of CRP were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human C-Reactive Protein/CRP Quantikine ELISA Kit
Human C-Reactive Protein/CRP ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: C-Reactive Protein/CRP
CRP binds and opsonizes apoptotic cells (6-8) as well as bacteria such as S. pneumoniae (9, 10).
It subsequently enhances the phagocytosis of these opsonized cells (6, 8-10). CRP additionally
binds several proteins in the complement cascade including C1q, C4BP, and Factor H (8, 11-13).
It enhances activation of the classical complement pathway and the deposition of C3b (9). In
later stages of the response, CRP inhibits complement-mediated cell lysis through its binding
to C4BP and Factor H (8, 12). These interactions induce the upregulation of complement
inhibitory proteins CD46, CD59, and CD55/DAF and inhibit assembly of the membrane attack
complex (MAC) (8, 14).
CRP binds to Fc gamma RI, Fc gamma RIIA, and Fc gamma RIIB on macrophages and dendritic cells (15-17), and
Fc receptors are required for the phagocytosis of CRP-opsonized target cells (6, 10, 18). CRP
binding to Fc gamma RI induces Src activation which subsequently triggers the inhibitory Fc gamma RIIb and
dampens the inflammatory response (15, 19). CRP additionally promotes dendritic cell
maturation and humoral immunity (10). In cardiovascular disease, CRP binds to oxidized LDL,
exacerbates tissue damage in coronary artery infarction, and inhibits the repair of injured
vascular endothelium (7, 19, 20).
Additional C-Reactive Protein/CRP Products
Product Documents for Human C-Reactive Protein/CRP Quantikine ELISA Kit
Product Specific Notices for Human C-Reactive Protein/CRP Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.