Human PD-1 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DPD10
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Cell Lysates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Urine (50 µL)
Sensitivity
3.27 pg/mL
Assay Range
15.6-1000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Product Summary for Human PD-1 Quantikine ELISA Kit
The Quantikine™ Human Programmed Death-1 (PD-1) Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human PD-1 in cell culture supernates, cell lysates, serum, plasma, and urine. It contains HEK293-expressed recombinant human PD-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human PD-1showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human PD-1.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human, Cynomolgus Monkey
Specificity
Natural and recombinant human PD-1.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision.
Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 125 | 319 | 655 | 131 | 304 | 626 |
| Standard Deviation | 3.49 | 5.60 | 10.8 | 7.36 | 14.6 | 25.8 |
| CV% | 2.6 | 1.8 | 1.6 | 5.6 | 4.8 | 4.1 |
Recovery for Human PD-1 Quantikine ELISA Kit
The recovery of human PD-1 spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 100 | 97-103 |
| Cell Lysis Buffer (n=1) | 96 | 94-97 |
| EDTA Plasma (n=4) | 86 | 81-93 |
| Heparin Plasma (n=4) | 85 | 75-93 |
| Serum (n=4) | 88 | 77-98 |
| Urine (n=4) | 89 | 83-97 |
Linearity
<div>To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human PD-1 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.</div>
Scientific Data Images for Human PD-1 Quantikine ELISA Kit
Human PD-1 ELISA Standard Curve
Human PD-1 Quantikine Recovery Competitor Comparison
PD-1 is spiked at three known concentrations throughout the range of the assay and run to measure response of the spiked sample matrix. Serum recovery is 95% compared to 136% for the top competitor. EDTA Plasma recovery is 87% compared to 157% for the top competitor. Culture media recovery is 94% compared to 58% for the top competitor. In spike and recovery experiments, natural samples are spiked with the recombinant target analyte of interest to identify interference caused by sample matrices.Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: PD-1
PD-1 expression is also regulated post-translationally (2). The PD-1 extracellular domain is
glycosylated at asparagine (N) residues N49 and N72 while in the endoplasmic reticulum. PD-1
subsequently transits to the golgi apparatus where it is fucosylated at the same sites by the
core fucosylase FUT8. This post translational modification represents a novel therapeutic target
as T cells have a more robust anticancer response due to reduced surface expression of
de-fucosylated PD-1. PD-1 is polyubiquinated at lysine (K) residue K48 by the E3 ligase F-Box 38
(FBX028) (2,7) which results in PD-1 degradation via the proteosome.
PD-1 serves as the receptor for programmed death ligand 1 (PD-L1/CD274/B7-H1) and PD-L2
(CD273/B7-DC) (8,9). The interaction of the extracellular domains of PD-1 and PD-L1 causes a
confirmational change that results in the phosphorylation of the cytoplasmic immunoreceptor
tyrosine-based inhibitory motif (ITIM) and the immunoreceptor tyrosine-based switch motif
(ITSM) by Src family kinases (4). Src homology 2 containing tyrosine phosphatase 2 (SHP-2) and
SHP-1 are recruited in order to attenuate T cell activating signals via dephosphorylation of
downstream signaling cascades.
Long Name
Programmed Death-1
Alternate Names
CD279, PD1, PDCD1, SLEB2
Entrez Gene IDs
Gene Symbol
PDCD1
Additional PD-1 Products
Product Documents for Human PD-1 Quantikine ELISA Kit
Product Specific Notices for Human PD-1 Quantikine ELISA Kit
For research use only
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