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Human Dkk-1 Quantikine ELISA Kit Best Seller

R&D Systems, part of Bio-Techne | Catalog # DKK100B

R&D Systems, part of Bio-Techne
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DKK100B

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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum-free Cell Culture Supernates (50 µL), Serum (10 µL), Platelet-poor EDTA Plasma (50 µL), Platelet-poor Heparin Plasma (50 µL)

Sensitivity

0.948 pg/mL

Assay Range

9.38-600 pg/mL (Cell Culture Supernates, Serum-free Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma)

Product Summary for Human Dkk-1 Quantikine ELISA Kit

The Quantikine® Human Dkk-1 immunoassay is a 4.5 hour solid phase ELISA designed to measure human Dkk-1 in cell culture supernates, serum-free cell culture supernates, serum, and platelet-poor plasma. It contains Sf 21-expressed recombinant human Dkk-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Dkk-1 showed dose-response curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values of natural human Dkk-1.

Product Specifications

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Reactivity

Human

Specificity

Natural and recombinant human Dkk-1.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.

Cell Culture Supernates, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Serum, Serum-free Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 75.6 187 360 75.6 192 372
Standard Deviation 2.21 3.28 9.48 5.81 16.7 30.1
CV% 2.9 1.8 2.6 7.7 8.7 8.1

Recovery for Human Dkk-1 Quantikine ELISA Kit

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 91 83-101
Platelet-poor EDTA Plasma (n=4) 95 91-98
Platelet-poor Heparin Plasma (n=4) 99 93-105
Serum-free Cell Culture Media (n=4) 82 77-90

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Dkk-1 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Human Dkk-1 ELISA Linearity
Human Dkk-1 ELISA Linearity

Scientific Data Images for Human Dkk-1 Quantikine ELISA Kit

Human Dkk-1 ELISA Cell Culture Supernate Standard Curve

Human Dkk-1 ELISA Cell Culture Supernate Standard Curve

Human Dkk-1 ELISA Standard Curve

Human Dkk-1 ELISA Standard Curve

Human Dkk-1 ELISA Serum/Plasma Standard Curve

Human Dkk-1 ELISA Serum/Plasma Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Dkk-1

Members of the dickkopf-related protein family (Dkk-1, -2, -3, and -4) are secreted proteins with two cysteine-rich domains separated by a linker region. Dkk-3 and -4 also have one prokineticin domain. Dkk-1, -2, and -4 function as antagonists of canonical Wnt signaling by binding to LRP5/6, preventing interaction of LRP5/6 with Wnt-Frizzled complexes. Dkk-1, -2, and -4 also bind cell surface Kremen-1 or -2 and promote the internalization of LRP5/6. Antagonistic activity of Dkk-3 has not been demonstrated. Dkk proteins have distinct patterns of expression in adult and embryonic tissues and have a wide range of effects on tissue development and morphogenesis. The Dkk family also includes Soggy, which is homologous to Dkk-3 but not to the other family members. Soggy has not been shown to inhibit Wnt signaling, and its role in the pathway is unclear.

Long Name

Dickkopf-1

Alternate Names

Dkk1

Entrez Gene IDs

22943 (Human); 13380 (Mouse); 102123735 (Cynomolgus Monkey)

Gene Symbol

DKK1

Additional Dkk-1 Products

Product Documents for Human Dkk-1 Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Dkk-1 Quantikine ELISA Kit

For research use only

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.