Human Galectin-1 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DGAL10
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Saliva (50 µL), Human Milk (50 µL)
Sensitivity
0.129 ng/mL
Assay Range
0.313-20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Human Milk)
Product Summary for Human Galectin-1 Quantikine ELISA Kit
The Quantikine Human Galectin-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Galectin-1 in cell culture supernates, serum, plasma, saliva, and human milk. It contains E. coli-expressed recombinant human Galectin-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Galectin-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Galectin-1.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Galectin-1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Human Milk, Saliva, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 1.96 | 4.66 | 8.51 | 2.07 | 5.02 | 9.37 |
| Standard Deviation | 0.139 | 0.408 | 0.483 | 0.197 | 0.444 | 0.701 |
| CV% | 7.1 | 8.8 | 5.7 | 9.5 | 8.8 | 7.5 |
Recovery for Human Galectin-1 Quantikine ELISA Kit
The recovery of Galectin-1 spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 102 | 93-115 |
| EDTA Plasma (n=4) | 96 | 87-102 |
| Heparin Plasma (n=4) | 93 | 80-102 |
| Serum (n=4) | 96 | 82-104 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of Galectin-1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Galectin-1 Quantikine ELISA Kit
Human Galectin-1 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Galectin-1
Alternate Names
BHL, Galaptin, Galectin1, GBP, L-14, LGALS1
Gene Symbol
LGALS1
Additional Galectin-1 Products
Product Documents for Human Galectin-1 Quantikine ELISA Kit
Product Specific Notices for Human Galectin-1 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.