Human IL-2 ELISA Kit - Quantikine
R&D Systems, part of Bio-Techne | Catalog # D2050
Key Product Details
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL), Citrate Plasma (100 µL)
Sensitivity
7 pg/mL
Assay Range
31.2-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Product Summary for Human IL-2 Quantikine ELISA Kit
The Quantikine Human IL-2 Immunoassay is a 4.5 hour solid phase ELISA designed to measure human IL-2 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-2 and antibodies raised against recombinant human IL-2. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IL-2 showed dose response curves that were parallel to the standard curves obtained using the recombinant Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-2.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human IL-2.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Serum/Plasma - Thirty-seven serum and plasma samples from apparently healthy volunteers
were evaluated for the presence of human IL-2 in this assay. All samples measured less than the
lowest Human IL-2 Standard, 31.3 pg/mL. No medical histories were available for the donors
used in this study.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) The recovery of human IL-2 spiked to three levels in samples throughout the range of the assay was evaluated.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 67.5 | 438 | 851 | 232 | 620 | 1148 |
| Standard Deviation | 2.9 | 14.4 | 26.9 | 23.2 | 34.4 | 45.5 |
| CV% | 4.3 | 3.3 | 3.2 | 10.0 | 5.5 | 4.0 |
Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 98.0 | 619 | 1183 | 250 | 755 | 1470 |
| Standard Deviation | 4.2 | 18.0 | 24.0 | 12.6 | 27.8 | 74.1 |
| CV% | 4.3 | 2.9 | 2.0 | 5.0 | 3.7 | 5.0 |
Recovery for Human IL-2 Quantikine ELISA Kit
The recovery of human IL-2 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=5) | 101 | 96-107 |
| Citrate Plasma (n=5) | 105 | 98-111 |
| EDTA Plasma (n=5) | 118 | 104-139 |
| Heparin Plasma (n=5) | 109 | 94-129 |
| Serum (n=5) | 99 | 89-114 |
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human IL-2 in various matrices and diluted with the appropriate calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human IL-2 Quantikine ELISA Kit
Human IL-2 ELISA Standard Curve for Cell Culture Supernate Assay
Human IL-2 ELISA Standard Curve for Serum/Plasma Assay
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-2
The receptor for IL-2 (IL-2 R) is composed of three subunits, the 55 kDa CD25/IL-2 R alpha chain, the
70 kDa IL-2 R beta chain, and the 65 kDa Common gamma Chain (1, 3). IL-2 first binds to CD25, the
binary complex then recruits IL-2 R beta and gammac to form the quaternary signaling complex (1, 14). In
addition to IL-2, IL-2 R beta is used by IL-15 in its quaternary signaling complex. gammac also serves as a
signaling receptor for IL-4, -7, -9, -15, and -21 (1, 3).
In vitro studies have shown an important role for IL-2 in T cell activation and expansion. In vivo,
IL-2 is critical for the development, maintenance and function of regulatory T cells (Treg) which
provide protection against autoimmune disease. On the other hand, IL-2 can also promote
autoimmune inflammation in target organs through its roles in regulating the expression of
T cell trafficking genes, and production of Th2 cytokines. Within the CD8+ T cell subset, IL-2 is
essential for optimal primary responses and differentiation into terminal effector cells. IL-2 also
promotes the development of activated CD8+ T cells into memory cells. (1).
Long Name
Interleukin 2
Alternate Names
Aldesleukin, IL2, Proleukin, TCGF
Entrez Gene IDs
Gene Symbol
IL2
Additional IL-2 Products
Product Documents for Human IL-2 Quantikine ELISA Kit
Product Specific Notices for Human IL-2 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.