Human IL-2 Quantikine HS ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # HS200
Key Product Details
Assay Length
4.0 hours
Sample Type & Volume Required Per Well
Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL)
Sensitivity
0.066 pg/mL
Assay Range
0.313-20 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human IL-2 Quantikine HS ELISA Kit
The Quantikine® HS Human IL-2 Immunoassay kit is a 4.0 hour solid phase ELISA designed to measure human IL-2 levels in serum and plasma. It contains
E. coli-expressed recombinant human IL-2 and antibodies raised against the recombinant factor. It has been shown to quantitate recombinant human IL-2 accurately. Results obtained using natural human IL-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine® HS kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-2.
E. coli-expressed recombinant human IL-2 and antibodies raised against the recombinant factor. It has been shown to quantitate recombinant human IL-2 accurately. Results obtained using natural human IL-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine® HS kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-2.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450 or 490nm (TMB or AP Substrate)
Reactivity
Human
Specificity
Natural and recombinant human IL-2.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 1.89 | 5.60 | 11.3 | 1.89 | 5.59 | 11.4 |
| Standard Deviation | 0.040 | 0.130 | 0.225 | 0.133 | 0.327 | 0.787 |
| CV% | 2.1 | 2.3 | 2.0 | 7.0 | 5.8 | 6.9 |
Recovery for Human IL-2 Quantikine HS ELISA Kit
The recovery of natural human IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 91 | 84-96 |
| Heparin Plasma (n=4) | 88 | 79-95 |
| Serum (n=4) | 91 | 86-94 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of natural human IL-2 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human IL-2 Quantikine HS ELISA Kit
Human IL-2 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-2
The receptor for IL-2 (IL-2 R) is composed of three subunits, the 55 kDa CD25/IL-2 R alpha chain, the 70 kDa IL-2 R beta chain, and the 65 kDa Common gamma Chain (1, 3). IL-2 first binds to CD25, the binary complex then recruits IL-2 R beta and gammac to form the quaternary signaling complex (1, 14). In addition to IL-2, IL-2 R beta is used by IL-15 in its quaternary signaling complex. gammac also serves as a signaling receptor for IL-4, -7, -9, -15, and -21 (1, 3).
In vitro studies have shown an important role for IL-2 in T cell activation and expansion. In vivo, IL-2 is critical for the development, maintenance and function of regulatory T cells (Treg) which provide protection against autoimmune disease. On the other hand, IL-2 can also promote autoimmune inflammation in target organs through its roles in regulating the expression of T cell trafficking genes, and production of Th2 cytokines. Within the CD8+ T cell subset, IL-2 is essential for optimal primary responses and differentiation into terminal effector cells. IL-2 also promotes the development of activated CD8+ T cells into memory cells. (1).
Long Name
Interleukin 2
Alternate Names
Aldesleukin, IL2, Proleukin, TCGF
Entrez Gene IDs
Gene Symbol
IL2
Additional IL-2 Products
Product Documents for Human IL-2 Quantikine HS ELISA Kit
Product Specific Notices for Human IL-2 Quantikine HS ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.