Human Soluble gp130 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DGP00
Key Product Details
Assay Length
4.5 hours or 5.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Citrate Plasma (10 µL)
Sensitivity
0.05 ng/mL (Cell Culture Supernates)
0.08 ng/mL (Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
0.08 ng/mL (Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Assay Range
0.125-8 ng/mL (Cell Culture Supernates)
0.25-16 ng/mL (Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
0.25-16 ng/mL (Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Product Summary for Human Soluble gp130 Quantikine ELISA Kit
The Quantikine Human soluble gp130 Immunoassay is a 4.5 or 5.5 hour solid phase ELISA designed to measure sgp130 in cell culture supernates, serum, and plasma. It contains soluble insect cell-expressed recombinant human gp130 and antibodies raised against recombinant human gp130 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural gp130 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural gp130.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human sgp130
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 0.40 | 1.00 | 4.43 | 0.40 | 1.02 | 4.50 |
| Standard Deviation | 0.02 | 0.07 | 0.33 | 0.02 | 0.10 | 0.31 |
| CV% | 5.0 | 7.0 | 7.4 | 5.0 | 9.8 | 6.9 |
Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 0.70 | 1.82 | 8.43 | 0.77 | 1.94 | 8.64 |
| Standard Deviation | 0.03 | 0.10 | 0.40 | 0.04 | 0.07 | 0.30 |
| CV% | 4.3 | 5.5 | 4.7 | 5.2 | 3.6 | 3.5 |
Recovery for Human Soluble gp130 Quantikine ELISA Kit
The recovery of sgp130 spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=5) | 109 | 96-114 |
| Citrate Plasma (n=5) | 98 | 84-112 |
| EDTA Plasma (n=5) | 100 | 83-114 |
| Heparin Plasma (n=5) | 98 | 85-110 |
| Serum (n=5) | 101 | 83-120 |
Linearity
To assess the linearity of the assay, five samples were spiked with high concentrations of sgp130 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Soluble gp130 Quantikine ELISA Kit
Human gp130 ELISA Serum/Plasma Standard Curve
Human gp130 ELISA Cell Culture Supernate Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: gp130
Gp130, the common signal transducing receptor component shared by the functional receptor complexes of the IL-6 family of cytokines, belongs to the classI cytokine receptor family. Binding of IL-6 or IL-11 to either the membrane-anchored or soluble IL-6 R or IL-11 R, respectively, initiates the association of IL-6 R (IL-11 R) with gp130. The complex then undergoes homodimerization and signal transduction. For other IL-6 family cytokines, such as LIF and OSM, signal transduction is similarly triggered by the heterodimerization of gp130 with LIFR or OSM R.
Long Name
Glycoprotein 130
Alternate Names
CD130, IL6ST
Gene Symbol
IL6ST
Additional gp130 Products
Product Documents for Human Soluble gp130 Quantikine ELISA Kit
Product Specific Notices for Human Soluble gp130 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.