Human Total Adiponectin/Acrp30 ELISA - Quantikine Best Seller
R&D Systems, part of Bio-Techne | Catalog # DRP300
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
Sensitivity
0.891 ng/mL
Assay Range
3.9-250 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human Total Adiponectin/Acrp30 Quantikine ELISA
The Quantikine Human Total Adiponectin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure total (low, middle, and high molecular weight) human Adiponectin in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human Adiponectin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Adiponectin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Adiponectin.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human total Adiponectin (low, middle, and high molecular weight)
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Serum/Plasma - Samples from apparently healthy volunteers were evaluated for the presence
of human Adiponectin in this assay. No medical histories were available for the donors used in
this study.
Cell Culture Supernates - Human peripheral blood leukocytes (1 x 106 cells/mL) were cultured in DMEM supplemented with 5% fetal bovine serum, 50 μM beta-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with 10 μg/mL PHA. Aliquots of the cell culture supernate were removed and assayed for levels of total human Adiponectin. No detectable levels were observed.
| Sample Type | Mean (ng/mL) | Range (ng/mL) | Standard Deviation (ng/mL) |
| Serum (n=60) | 6641 | 865 - 21,424 | 3665 |
| EDTA plasma (n=35) | 5548 | 1198 - 19,973 | 3557 |
| Heparin plasma (n=35) | 6026 | 1359 - 20,691 | 3728 |
Cell Culture Supernates - Human peripheral blood leukocytes (1 x 106 cells/mL) were cultured in DMEM supplemented with 5% fetal bovine serum, 50 μM beta-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with 10 μg/mL PHA. Aliquots of the cell culture supernate were removed and assayed for levels of total human Adiponectin. No detectable levels were observed.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (ng/mL) | 12.5 | 45.3 | 91.5 | 12.7 | 47.1 | 100 |
| Standard Deviation | 0.41 | 1.27 | 4.56 | 1.00 | 2.80 | 6.70 |
| CV% | 3.2 | 2.8 | 5.0 | 7.9 | 5.9 | 6.7 |
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (ng/mL) | 19.8 | 69.9 | 143 | 20.5 | 74.4 | 157 |
| Standard Deviation | 0.50 | 2.40 | 6.76 | 1.40 | 4.30 | 10.8 |
| CV% | 2.5 | 3.4 | 4.7 | 6.8 | 5.8 | 6.9 |
Recovery for Human Total Adiponectin/Acrp30 Quantikine ELISA
The recovery of Adiponectin spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 101 | 88-112 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Adiponectin were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Total Adiponectin/Acrp30 Quantikine ELISA
Human Total Adiponectin ELISA/Acrp30 ELISA Cell Culture Supernate Standard Curve
Human Total Adiponectin ELISA/Acrp30 ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Adiponectin/Acrp30
Injection of Adiponectin into non-obese diabetic mice leads to an insulin-independent decrease in
glucose levels (11). This is likely due to insulin-sensitizing effects involving Adiponectin regulation of
triglyceride metabolism (11). A truncated form of Adiponectin (gAdiponectin) containing only the
C-terminal globular domain has been identified in the blood, and recombinant gAdiponectin has
been shown to regulate weight reduction as well as free fatty acid oxidation in mouse muscle and
liver (2, 12). The full-length recombinant Adiponectin protein is apparently less potent at mediating
these effects (2, 12). The mechanism underlying the role of Adiponectin in lipid oxidation may
involve the regulation of expression or activity of proteins associated with triglyceride metabolism
including CD36, acyl CoA oxidase, AMPK, and PPAR gamma (12-14).
Although Adiponectin-regulation of glucose and lipid metabolism in humans is less clear, similar
mechanisms may also be in place (15). A negative correlation between obesity and circulating
Adiponectin has been well established (6, 16, 17), and Adiponectin levels increase concomitantly
with weight loss (18). Decreased Adiponectin levels are associated with insulin resistance and
hyperinsulinemia, and patients with type-2 diabetes are reported to exhibit decreased circulating
Adiponectin (19, 20). Thiazolidinediones, a class of insulin-sensitizing, anti-diabetic drugs, elevate
Adiponectin in insulin-resistant patients (21). In addition, high Adiponectin levels are associated
with a reduced risk of type-2 diabetes (22). Using magnetic resonance spectroscopy it has
been demonstrated that intracellular lipid content in human muscle negatively correlates with
Adiponectin levels, potentially due to Adiponectin-induced fatty acid oxidation (15).
Adiponectin may also play anti-atherogenic and anti-inflammatory roles. Adiponectin plasma levels
are decreased in patients with coronary artery disease (20). Furthermore, neointimal thickening
of damaged arteries is exacerbated in Adiponectin-deficient mice and is inhibited by exogenous
Adiponectin (23). Adiponectin inhibits endothelial cell expression of adhesion molecules in vitro,
suppressing the attachment of monocytes (24). In addition, Adiponectin negatively regulates
myelomonocytic progenitor cell growth and TNF-alpha production in macrophages (25, 26).
Alternate Names
Acrp30, AdipoQ, ApM1, GBP28
Gene Symbol
ADIPOQ
Additional Adiponectin/Acrp30 Products
Product Documents for Human Total Adiponectin/Acrp30 Quantikine ELISA
Product Specific Notices for Human Total Adiponectin/Acrp30 Quantikine ELISA
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.