Human Total Serpin E1/PAI-1 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DTSE100
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Citrate Plasma (10 µL)
Sensitivity
0.046 ng/mL
Assay Range
0.313-20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Product Summary for Human Total Serpin E1/PAI-1 Quantikine ELISA Kit
The Quantikine Human Total Serpin E1/PAI-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Serpin E1 in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human Serpin E1 and has been shown to accurately quantitate the recombinant factor free and in complex with uPA, tPA, or vitronectin. Results obtained using natural human Serpin E1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Serpin E1.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Total Serpin E1 (active, latent, uPA, tPA, and Vitronectin complexed Serpin E1).
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 1.61 | 5.05 | 11.1 | 1.82 | 5.28 | 10.0 |
| Standard Deviation | 0.097 | 0.330 | 0.495 | 0.196 | 0.451 | 0.604 |
| CV% | 6.0 | 6.5 | 4.5 | 10.8 | 8.5 | 6.0 |
Recovery for Human Total Serpin E1/PAI-1 Quantikine ELISA Kit
The recovery of human Total Serpin E1 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 105 | 90-119 |
| Citrate Plasma (n=4) | 104 | 87-118 |
| EDTA Plasma (n=4) | 99 | 85-113 |
| Heparin Plasma (n=4) | 103 | 81-116 |
| Serum (n=4) | 104 | 93-116 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of human Serpin E1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. All samples were diluted prior to assay.
Scientific Data Images for Human Total Serpin E1/PAI-1 Quantikine ELISA Kit
Human Total Serpin E1/PAI-1 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Serpin E1/PAI-1
Long Name
Plasminogen Activator Inhibitor
Alternate Names
Nexin, PAI-1, PAI1, PLANH1
Gene Symbol
SERPINE1
Additional Serpin E1/PAI-1 Products
Product Documents for Human Total Serpin E1/PAI-1 Quantikine ELISA Kit
Product Specific Notices for Human Total Serpin E1/PAI-1 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.