Human IFN-gamma ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91174

Novus Biologicals, part of Bio-Techne
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NBP1-91174
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma (EDTA, citrate, heparin), buffered solution (50 uL)

Sensitivity

0.99 pg/mL

Assay Range

1.6 - 100 pg/mL

View all IFN-gamma ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human IFN gamma positive serum. There was no crossreactivity detected.

Description

The human IFN-gamma ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IFN-gamma.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.50%

Inter-Assay Precision (Precision between assays) CV% < 5.70%

Recovery for Human IFN-gamma ELISA Kit (Colorimetric)

Recovery

The recovery ranged from 88% to 112% with an overall mean recovery of 97%. Please see protocol for more specific Spike Recovery details.

Scientific Data Images for Human IFN-gamma ELISA Kit (Colorimetric)

ELISA: Human IFN-gamma ELISA Kit (Colorimetric) [NBP1-91174]

ELISA: Human IFN-gamma ELISA Kit (Colorimetric) [NBP1-91174]

ELISA: IFN-gamma ELISA Kit [NBP1-91174] - Standard curve

Kit Contents for Human IFN-gamma ELISA Kit (Colorimetric)

  • * reagent contains ethyl alcohol
  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human IFN-gamma
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Biotin-Conjugate anti-human IFN-gamma monoclonal antibody
  • Human IFN-gamma Standard lyophilized, 200 ng/mL upon reconstitution
  • Sample Diluent
  • Stop Solution (1M Phosphoric acid)
  • Streptavidin-HRP
  • Substrate Solution
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Formulation

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IFN-gamma

IFN-gamma, also called Type II interferon, is a homodimeric glycoprotein containing approximately 21 to 24 kD subunits. The human IFN-gamma gene, situated on chromosome 12, contains three introns; the four exons code for a polypeptide of 166 amino acids, 20 of which constitute the signal peptide. In contrast to IFN-alpha and IFN-beta synthesis, which can occur in any cell, production of IFN-gamma is a function of T cells and NK cells. IFN-gamma has antiviral and antiparasitic activities and also inhibits the proliferation of a number of normal and transformed cells. IFN-gamma synergises with TNF-alpha and TNF-beta in inhibiting the proliferation of various cell types. DetailsDocumentationAdditional FormatsReferences

Long Name

Interferon gamma

Alternate Names

IFG, IFI, IFNG, IFNgamma

Gene Symbol

IFNG

Additional IFN-gamma Products

Product Documents for Human IFN-gamma ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human IFN-gamma ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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