Rat IFN-gamma ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92680

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum (50 uL)

Sensitivity

9.9 pg/mL

Assay Range

31.3 - 2,000 pg/mL

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

The interference of circulating factors of the immune systemwas evaluated by spiking these proteins at physiologically relevant concentrations into a rat IFN gammapositive serum. There was no crossreactivity detected.

Description

The rat IFN-gamma ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of rat IFN-gamma

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.0%

Inter-Assay Precision (Precision between assays) CV% < 10%

Recovery for Rat IFN-gamma ELISA Kit (Colorimetric)

Recovery

Refer to manual

Scientific Data Images for Rat IFN-gamma ELISA Kit (Colorimetric)

ELISA: Interferon gamma ELISA Kit 96Tests [NBP1-92680]

Kit Contents for Rat IFN-gamma ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to rat IFN-gamma
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Biotin-Conjugate anti-rat IFN-gamma monoclonal antibody
  • Rat IFN-gamma Standard lyophilized, 4 ng/ml upon reconstitution
  • Sample Diluent
  • Stop Solution (1M Phosphoric acid)
  • Streptavidin-HRP
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IFN-gamma

IFN-gamma, also called Type III interferon, is a homodimeric glycoprotein containing approximately 21 to 24 kD subunits. In contrast to IFN-alpha and IFN-beta synthesis, which can occur in any cell, production of IFN-gamma is a function of T cells and NK cells. All IFN-gamma inducers activate T cells either in a polyclonal (mitogens or antibodies) or in a clonally restricted, antigen-specific, manner. IFN-gamma is produced during infection by T cells of the cytotoxic/suppressor phenotype (CD8) and by a subtype of helper T cells, the Th1 cells. Th1 cells secrete IL-2, IL-3, TNF-beta and IFN-gamma, whereas Th2 cells mainly produce IL-3, IL-4, IL-5, and IL-10, but little or no IFN-gamma. IFN-gamma preferentially inhibits the proliferation of Th2 but not Th1 cells, indicating that the presence of IFN-gamma during an immune response will result in the preferential proliferation of Th1 cells. Type II IFN or IFN-gamma is a lymphokine that displays no molecular homology with type I IFN, but shares some important biologic activities. Specifically, IFN-gamma induces an anti-viral state and is anti-proliferative. In addition, IFN-gamma has several properties related to immunoregulation. (1) IFN-gamma is a potent activator of mononuclear phagocytes, augments endocytosis and phagocytosis by monocytes, and activates macrophages to kill tumor cells by releasing reactive oxygen intermediates and TNF-alpha. (2) IFN-gamma induces or augments the expression of MHC antigens on macrophages, T and B cells and some tumor cell lines. (3) On T and B cells IFN-gamma promotes differentiation. (4) Finally, IFN-gamma activates neutrophils, NK cells and vascular endothelial cells.

Long Name

Interferon gamma

Alternate Names

IFG, IFI, IFNG, IFNgamma

Gene Symbol

IFNG

Additional IFN-gamma Products

Product Documents for Rat IFN-gamma ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Rat IFN-gamma ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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