IkB-alpha Antibody Pair [HRP]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-79383

Novus Biologicals, part of Bio-Techne
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Key Product Details

Assay Type

Sandwich ELISA

Assay Range

12.5-800 pg/ml (example only; lot dependent)

Sensitivity

12.5 pg/ml (example only; lot dependent)

Reactivity

Human

View all IkB-alpha ELISA Kits »

Product Specifications

Description

Solid Phase sandwich ELISA for the quantitative determination of Human IkB-alpha.

Sample Volume Required

100 ul

Conjugate

HRP

Scientific Data Images for IkB-alpha Antibody Pair [HRP]

Sandwich ELISA: IkB-alpha Antibody Pair [HRP] [NBP2-79383] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

Kit Contents for IkB-alpha Antibody Pair [HRP]

  • Mouse Monoclonal Detection Antibody (HRP-conjugated)
  • Rabbit Monoclonal Capture Antibody: (Catalog # NBP2-90042)
  • Standard

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IkB-alpha

NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified (1). One of the first gene induced following NF-kB activation is IkBa.

Long Name

I-kappa-B-alpha

Alternate Names

IkBalpha, MAD-3, NFKBIA

Gene Symbol

NFKBIA

Additional IkB-alpha Products

Product Documents for IkB-alpha Antibody Pair [HRP]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IkB-alpha Antibody Pair [HRP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

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