Human IL-2 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89875

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma (EDTA, citrate, heparin) (50 uL)

Sensitivity

9.9 pg/mL

Assay Range

18.8 - 1,200 pg/mL

View all IL-2 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

The cross reactivity and interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human IL-2 positive serum. There was no cross reactivity or interference detected.

Description

This kit is an enzyme-linked immunosorbent assay for quantitative detection.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 7.0%

Inter-Assay Precision (Precision between assays) CV% < 5.0%

Recovery for Human IL-2 ELISA Kit (Colorimetric)

Recovery

See protocol for Spike recovery results.

Scientific Data Images for Human IL-2 ELISA Kit (Colorimetric)

ELISA: Human IL-2 ELISA Kit (Colorimetric) [NBP1-89875]

ELISA: Human IL-2 ELISA Kit (Colorimetric) [NBP1-89875]

ELISA: IL-2 ELISA Kit (Colorimetric) [NBP1-89875] - Representative standard curve for human IL-2 ELISA. Human IL-2 was diluted in serial 2-fold steps in SampleDiluent. Do not use this standard curve to derive test results. A standard curve must be run for each group ofmicrowell strips assayed.

Kit Contents for Human IL-2 ELISA Kit (Colorimetric)

  • Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human BTLA

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-2

IL-2 plays a central role in the activation and proliferation of lymphocytes that have been primed by antigens. IL-2 plays a pivotal role in for the expansion of most T-cells, natural killer cells and B-cells during certain phases of their response. IL-2 is a 15 kDa glycoprotein encoded by a single gene located in the q26-28 region of human chromosome 4. The cDNA deduced polypeptide consists of 153 amino acids. Apart from its most important role to mediate antigen-specific T-lymphocyte proliferation, IL-2 modulates the expression of interferon gamma and major histocompatibility antigens, stimulates proliferation and differentiation of activated B-cells, augments natural killer cell activity and inhibits granulocyte-macrophage colony formation. IL-2 displays antitumoral effects, thus being used in cancer therapy. Monitoring of IL-2 levels in serum provides more detailed insights in several pathological situations such as cancer, infectious diseases, transplant rejection, multiple sclerosis, rheumatoid arthritis, systemic lupus erythematosus and type I diabetes.

Long Name

Interleukin 2

Alternate Names

Aldesleukin, IL2, Proleukin, TCGF

Gene Symbol

IL2

Additional IL-2 Products

Product Documents for Human IL-2 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human IL-2 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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