Mouse IL-2 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92661

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum (50 uL)

Sensitivity

5.3 pg/mL

Assay Range

15.6 - 1,000 pg/mL

View all IL-2 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Specificity

The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a mouse IL-2 positive serum. There was no crossreactivity detected.

Description

The mouse IL-2 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse IL-2.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.00%

Inter-Assay Precision (Precision between assays) CV% < 0.1

Recovery for Mouse IL-2 ELISA Kit (Colorimetric)

Recovery

119%

Scientific Data Images for Mouse IL-2 ELISA Kit (Colorimetric)

IL-2 ELISA Kit [NBP1-92661] - Data for Mouse IL-2 Platinum ELISA

Kit Contents for Mouse IL-2 ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse sCD40L
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Biotin-Conjugate anti-mouse sCD40L monoclonal antibody
  • Mouse sCD40L Standard lyophilized, 40 ng/ml upon reconstitution
  • Sample Diluent
  • Stop Solution (1M Phosphoric acid)
  • Streptavidin-HRP
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-2

Interleukin-2 (IL-2) plays a central role in the activation and proliferation of lymphocytes that have been primed by antigens. IL-2 plays a pivotal role in for the expansion of most T-cells, natural killer cells and B-cells during certain phases of their response. Antigen-specific proliferation of helper and cytotoxic T-lymphocytes following stimulation is critically dependent on IL-2 expression, secretion, and binding to receptors in an autocrine fashion on the surface of T cells. Apart from its most important role to mediate antigen specific T lymphocyte proliferation, IL-2 modulates the expression of interferon gamma and major histocompatibility antigens, stimulates proliferation and differentiation of activated B and NK cells, augments natural killer cell activity and inhibits granulocyte-macrophage colony formation. Alterations in the ability of T cells to synthesize IL-2 have been observed in physiologic and pathologic states.

Long Name

Interleukin 2

Alternate Names

Aldesleukin, IL2, Proleukin, TCGF

Gene Symbol

IL2

Additional IL-2 Products

Product Documents for Mouse IL-2 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mouse IL-2 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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