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Rat LDLR ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81149

Novus Biologicals, part of Bio-Techne
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NBP2-81149

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

0.19 ng/mL

Assay Range

0.31 - 20 ng/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat LDLR in samples. No significant cross-reactivity or interference between Rat LDLR and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.87%

Inter-Assay Precision (Precision between assays) CV% < 5.53%

Recovery for Rat LDLR ELISA Kit (Colorimetric)

Recovery

89-109%

Linearity

Rat LDLR ELISA Kit (Colorimetric)

Scientific Data Images for Rat LDLR ELISA Kit (Colorimetric)

Rat LDLR ELISA Kit (Colorimetric)

ELISA: Rat LDLR ELISA Kit (Colorimetric) [NBP2-81149] -

ELISA: Rat LDLR ELISA Kit (Colorimetric) [NBP2-81149] - Standard Curve Reference

Kit Contents for Rat LDLR ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: LDLR

The low density lipoprotein (LDL) receptor system coordinates the metabolism of cholesterol, an essential component of the plasma membrane of all mammalian cells. Study of this system has led to an enhanced understanding of the cellular basis of cholesterol homeostasis. It has also brought into focus an important mechanism of metabolic regulation--the process of receptor-mediated endocytosis. Data suggest that the juxtamembranous region of the cytoplasmic domain participates in protein:protein interactions that allow the low density lipoprotein receptor to cluster in coated pits. It has been shown that the family of LDL receptors may serve as viral receptors. Endocytosis of the Flaviviridae viruses, hepatitis C virus, GB virus C/hepatitis G virus, and bovine viral diarrheal virus (BVDV) were shown to be mediated by LDL receptors on cultured cells.

Long Name

Low Density Lipoprotein Receptor

Alternate Names

LDL R

Gene Symbol

LDLR

Additional LDLR Products

Product Documents for Rat LDLR ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat LDLR ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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