Mouse Adiponectin/Acrp30 Quantikine ELISA Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # MRP300
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Tissue Homogenates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
Sensitivity
0.007 ng/mL
Assay Range
0.16-10 ng/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Mouse Adiponectin/Acrp30 Quantikine ELISA Kit
The Quantikine Mouse Adiponectin Immunoassay is a 4.5 hour solid phase ELISA designed to measure full-length mouse Adiponectin levels in cell culture supernates, tissue homogenates, serum, and plasma. It contains NS0-expressed recombinant mouse Adiponectin and antibodies raised against the recombinant protein. Results obtained for naturally occurring mouse Adiponectin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural mouse Adiponectin.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant full-length mouse Adiponectin. This assay only recognizes full-length mouse Adiponectin. It does not recognize mouse gAdiponectin.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Tissue Homogenates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 23 | 23 | 23 |
| Mean (ng/mL) | 0.33 | 1.23 | 3.83 | 0.35 | 1.22 | 4.02 |
| Standard Deviation | 0.022 | 0.072 | 0.221 | 0.021 | 0.061 | 0.258 |
| CV% | 6.7 | 5.9 | 5.8 | 6.0 | 5.0 | 6.4 |
Recovery for Mouse Adiponectin/Acrp30 Quantikine ELISA Kit
The recovery of mouse Adiponectin spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=7) | 103 | 82-115 |
| EDTA Plasma (n=4) | 92 | 81-106 |
| Heparin Plasma (n=4) | 93 | 77-110 |
| Serum (n=6) | 98 | 85-114 |
Linearity
To assess the linearity of the assay, samples containing mouse Adiponectin in each matrix were diluted with Calibrator Diluent and then assayed.
Scientific Data Images for Mouse Adiponectin/Acrp30 Quantikine ELISA Kit
Mouse Adiponectin/Acrp30 ELISA Standard Curve
Mouse Adiponectin/Acrp30 Quantikine ELISA Kit
Changes in steroid hormone levels and adipokine levels in peripheral blood. (a) Testosterone. (b) Estradiol. (c) Corticosterone. (d) DHEA. (e) Leptin. (f) Adiponectin. For all groups, n = 8. Values are expressed as mean ± standard error. *P < 0.05, significantly different from the 3moR group. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41598-017-12358-8), licensed under a CC-BY license. Not internally tested by R&D Systems.Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Adiponectin/Acrp30
Adiponectin (also known as Adipocyte complement-related protein of 30 kDa, Acrp30) is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. Adiponectin associates into 70 kDa noncovalently-linked trimers that are disulfide-linked into medium molecular weight hexamers and then into >300 kDa high molecular weight oligomers.
Alternate Names
Acrp30, AdipoQ, ApM1, GBP28
Gene Symbol
ADIPOQ
Additional Adiponectin/Acrp30 Products
Product Documents for Mouse Adiponectin/Acrp30 Quantikine ELISA Kit
Product Specific Notices for Mouse Adiponectin/Acrp30 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.