Mouse Angiopoietin-like 4 DuoSet ELISA

Name: Mouse Angiopoietin-like 4 DuoSet ELISA
Brand: R&D Systems
SKU: DY4880-05

R&D Systems, part of Bio-Techne | Catalog # DY4880-05

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Product Datasheet / COA / SDS

Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	0.313 - 20 ng/mL
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Mouse Angiopoietin-like 4 DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

View Full Technical Details for Mouse Angiopoietin-like 4 DuoSet ELISA

Technical Data
Citations / Reviews

Summary for Mouse Angiopoietin-like 4 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse ANGPTL4. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Specifications for Mouse Angiopoietin-like 4 DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	100 µL
Reactivity	Mouse
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For five 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Mouse Angiopoietin-like 4 DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates):(Catalog #DY008B) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS:(Catalog #DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer:(Catalog #WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: ELISA TMB Substrate (R&D Systems,

Catalog #DY999B)

Stop Solution:2N H2SO4(Catalog #DY994)

Microplates: R&D Systems (Catalog #DY990)

Plate Sealers:ELISA Plate Sealers (Catalog #DY992)

*For the Reagent Diluent and Blocking Buffer arerecommended for a specific DuoSet ELISA Development Kit,see the product datasheet

Scientific Data Examples for Mouse Angiopoietin-like 4 DuoSet ELISA

	Mouse ANGPTL4 DuoSet ELISA Standard Curve

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Angiopoietin-like Protein 4/ANGPTL4

Angiopoietin-like, also known as angiopoietin-related proteins, are structurally related to the angiopoietins. They are secreted proteins with N-terminal coiled-coil domains and C-terminal fibrinogen-like domains. Unlike the angiopoietins, ANGPTLs do not bind Tie-2. Seven ANGPTL proteins have been identified (ANGPTL1 through 7).

Entrez Gene IDs

51129 (Human); 57875 (Mouse); 362850 (Rat)

Alternate Names

ANGPTL4, ARP4fasting-induced adipose factor, Angiopoietin like Protein 4, Angiopoietin-like Protein 4, FIAF, FIAFhepatic angiopoietin-related protein, HFARP, HFARPANGPTL2, Hepatic fibrinogen/angiopoietin-related protein, NL2, PGAR, PGARangiopoietin-related protein 4, PPARG angiopoietin related protein, angiopoietin-like 4, peroxisome proliferator-activated receptor (PPAR) gamma inducedangiopoietin-related protein, pp1158

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