Human NF-L ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81184

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

9.38 pg/mL

Assay Range

15.63 - 1000 pg/mL

View all NF-L ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human NF-L in samples. No significant cross-reactivity or interference between Human NF-L and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.94%

Inter-Assay Precision (Precision between assays) CV% < 4.92%

Recovery for Human NF-L ELISA Kit (Colorimetric)

Recovery

86-106%

Linearity

Human NF-L ELISA Kit (Colorimetric)

Scientific Data Images for Human NF-L ELISA Kit (Colorimetric)

Human NF-L ELISA Kit (Colorimetric)

ELISA: Human NF-L ELISA Kit (Colorimetric) [NBP2-81184] -

ELISA: Human NF-L ELISA Kit (Colorimetric) [NBP2-81184] - Standard Curve Reference

Kit Contents for Human NF-L ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: NF-L

Neurofilaments are 10nm intermediate filament proteins located in vertebrate neurons, which regulate axonal diameter. They are composed predominantly of the three major neurofilament proteins: NF-Light, NF-Medium and NF-Heavy. NF-L is the light or low molecular weight polypeptide, and it runs on SDS-PAGE gels at about 68kDa, with some size variation in across species.

Antibodies to NF-L may be used to identify NF-L in neurons and to study neuronal processes in tissue sections and/or culture. NF-L antibodies can also be used to study microfilament accumulations seen in many neurological diseases, such as Lou Geri's disease or Alzheimer's disease.

Long Name

Neurofilament Protein, Light Chain

Alternate Names

CMT2E, NEFL, NF68, NFL

Gene Symbol

NEFL

Additional NF-L Products

Product Documents for Human NF-L ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human NF-L ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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