Porcine IL-1 beta/IL-1F2 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # PLB00B
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 µL), Serum (50 µL), Heparin Plasma (50 µL)
Sensitivity
13.6 pg/mL
Assay Range
39.1-2500 pg/mL (Cell Culture Supernates, Serum, Heparin Plasma)
Product Summary for Porcine IL-1 beta/IL-1F2 Quantikine ELISA Kit
The Quantikine Porcine IL-1 beta Immunoassay is a 4.5 hour solid phase ELISA designed to measure porcine IL-1 beta in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mature porcine IL-1 beta and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant porcine IL-1 beta. Results obtained using natural porcine IL-1 beta show dose response curves that are parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural porcine IL-1 beta.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Porcine
Specificity
Natural and recombinant porcine IL-1 beta
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 136 | 348 | 780 | 141 | 338 | 783 |
| Standard Deviation | 9.8 | 17.0 | 40.9 | 12.2 | 20.5 | 31.7 |
| CV% | 7.2 | 4.9 | 5.2 | 8.7 | 6.1 | 4.0 |
Recovery for Porcine IL-1 beta/IL-1F2 Quantikine ELISA Kit
The recovery of porcine IL-1 beta spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 106 | 102-109 |
| Heparin Plasma (n=4) | 111 | 98-117 |
| Serum (n=4) | 111 | 102-117 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with various concentrations of porcine IL-1 beta in each matrix were diluted with Calibrator Diluent and then assayed.
Scientific Data Images for Porcine IL-1 beta/IL-1F2 Quantikine ELISA Kit
Porcine IL-1 beta/IL-1F2 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-1 beta/IL-1F2
Long Name
Interleukin 1 beta
Alternate Names
IL-1b, IL-1F2, IL1 beta, IL1B
Entrez Gene IDs
Gene Symbol
IL1B
Additional IL-1 beta/IL-1F2 Products
Product Documents for Porcine IL-1 beta/IL-1F2 Quantikine ELISA Kit
Product Specific Notices for Porcine IL-1 beta/IL-1F2 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.