BOP-JF646
Tocris Bioscience, part of Bio-Techne | Catalog # 6997
Key Product Details
| Description: | Red fluorescent dual α9β1/α4β1 integrin inhibitor comprising BOP conjugated to Janelia Fluor® 646; fluorogenic; photostable |
| Chemical Name: | 2-(3-(Azetidin-1-ium-1-ylidene)-7-(azetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)-5-(((1-((3R,5S)-5-(((S)-1-carboxy-2-(4-((pyrrolidine-1-carbonyl)oxy)phenyl)ethyl)carbamoyl)-1-(phenylsulfonyl)pyrrolidin-3-yl)-1H-1,2,3-triazol-4-yl)methyl)carbamoyl)benzoate |
| Purity: | ≥90% (HPLC) |
| Molecular Weight: | 1090.3 |
Biological Activity for BOP-JF646
BOP-JF646 is a fluorescent dual α9β1/α4β1 integrin inhibitor. Comprises BOP (Cat. No. 6047) conjugated to Janelia Fluor® 646 (Cat. No. 6148). Fluorogenic: fluoresces only once bound to integrins, enabling hassle-free no-wash experiments. Bright and photostable, enabling live cell tracking of integrin receptors over long time-course experiments. Excitation maximum = 655 nm; emission maximum = 672 nm.
Optical Data for BOP-JF646
| Emission Color | Red |
|---|---|
| Brightness | |
| λabs | 655 nm |
| λem | 672 nm |
| Closest Laser line | 647 nm |
| Application | Flow Cytometry, Confocal Microscopy, Super Resolution Microscopy (SRM) |
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Application of BOP-JF549 in Immortalized Human Keratinocyte Cells. Immortalized human keratinocytes expressing a GFP-tagged alpha 4 integrin were incubated with 10 μm BOP-Janelia Fluor® 568. Cells were incubated at 37°C and imaged using Nikon A1R confocal microscope. Scale bar = 10 μm.Image kindly provided by Willow Hight-Warburton; Kings College London. |
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