Flutax 2
Tocris Bioscience, part of Bio-Techne | Catalog # 6254
Key Product Details
| Description: | Green-fluorescent taxol derivative; binds microtubules. Used for: imaging microtubules in live cells, isolated cytoskeletons and microtubule suspensions |
| Chemical Name: | (2aR,4S,4aS,6R,9S,11S,12S,12aR,12bS)-6,12b-Bis(acetyloxy)-9-[(2R,3S)-3-(benzoylamino)-2-hydroxy-1-oxo-3-phenylpropoxy]-12-(benzoyloxy)-2a,3,4,4a,5,6,9,10,11,12,12a,12b-dodecahydro-11-hydroxy-4a,8,13,13-tetramethyl-5-oxo-7,11-methano-1H-cyclodeca[3,4]benz[1,2-b]oxet-4-yl ester N-[(2',7'-difluoro-3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)carbonyl]-L-alanine |
| Purity: | ≥90% (HPLC) |
| Molecular Weight: | 1319.28 |
Biological Activity for Flutax 2
Key information: Flutax 2 is a green-fluorescent taxol derivative; for microtubules labeling in live cells.
Used for: imaging microtubules in live cells, isolated cytoskeletons and microtubule suspensions.
Application: fluorescence microscopy.
Properties and Photophysical Data: The fluorescent label on Flutax 2 is attached by derivatization of the 7-β-hydroxy group of native paclitaxel which permits selective binding of the probe to microtubules. Flutax 2 binds microtubules with high affinity (Ka ~ 107M-1). Flutax 2 binds to α, β tubulin dimers and stabilizes them. Flutax 2 is soluble and pH insensitive and is more photostable than Flutax 1 (Cat. No. 2226). Excitation and emission maxima (λ) are 496 nm and 526 nm, respectively.
Flutax 2-labeled HeLa Cells
Optical Data for Flutax 2
| λabs | 496 nm |
|---|---|
| λem | 526 nm |
| Extinction Coefficient (ε) | 80000 M-1cm-1 |
| Quantum Yield (φ) | 0.9 |
| Application | Fluorescence Microscopy |
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Flutax 2 labeled HeLa cells. Flutax 2 was diluted in HBSS and applied to live HeLa cells at a concentration of 2 μM. HeLa cells were incubated for 1 hour at 37°C. After incubation was complete, Flutax 2 was washed off, and fresh HBSS was applied to the cells. Cells were not fixed because Flutax 2 staining is not retained at all after fixation (photobleach). Like Flutax 1, Flutax 2 staining in live cells diminishes very rapidly when exposed to light, so the cells should be exposed to light as little as possible to retain good signal. |
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