Janelia Fluor® 646b, Maleimide
Tocris Bioscience, part of Bio-Techne | Catalog # 8158
Key Product Details
| Description: | Blinking fluorescent dye (Reactive group: maleimide). Application: super resolution microscopy (SRM) including SMLM |
| Chemical Name: | 3,7-Di(azetidin-1-yl)-N-(2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)-5,5-dimethyl-3'H,5H-spiro[dibenzo[b,e]siline-10,1'-isobenzofuran]-6'-carboxamide |
| Purity: | ≥90% (HPLC) |
| Molecular Weight: | 604.78 |
Biological Activity for Janelia Fluor® 646b, Maleimide
Spontaneously Blinking Janelia Fluor® Dyes allow facile single-molecule localization microscopy (SMLM) in cells and dense biomolecular structures, without the need for photoactivation or redox buffers. These spontaneously blinking dyes harness Janelia Fluor® technology to delivery dyes that automatically cycle between 'off' and 'on' states with an ideal duty cycle for super resolution microscopy experiments.
Key Information: Janelia Fluor® 646b, Maleimide is a spontaneously blinking fluorescent dye, supplied with a maleimide reactive group for conjugation (thiol reactivity) for the labeling of protein. Suitable to characterize the physical properties of dense biomolecule condensates and complex macromolecular assemblies.
Application: suitable for super resolution microscopy (SRM) including single-molecule localization microscopy (SMLM).
Properties and Photophysical Data: derived from Janelia Fluor® 646, Maleimide (Cat. No. 6590). Excitation and emission maxima (λ) are 662 nm and 674 nm, respectively.
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Janelia Fluor® 646b, Maleimide
| λabs | 662 nm |
|---|---|
| λem | 674 nm |
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